Cultivation method for worm grass containing high amount of cordycepin and growth medium used by method

Abstract

The invention discloses a cultivation method for worm grass containing high amount of cordycepin and a growth medium used by a method. The method comprises following steps: (a) making the growth medium, wherein components and mass percents of components of the growth medium are as follows: 75%-100% of egg powder, 0%-20% of maltodextrin, 0%-5% of adenosine; wherein the sum of the components for the growth medium is 100%; (b) inoculating cordyceps militaris strains on the growth medium; (c) harvesting and obtaining required cordyceps militaris under cultivation in a certain environment condition. The cultivation method for worm grass containing a high amount of cordycepin and the growth medium used by the method have following beneficial effects: production efficiency of cordyceps militaris is increased so that production cycle is shortened; production equipment is easy; production condition is easy to control; cost of manufactured production is low; and amount of cordycepin for worm grass is up to 1.5-4.0%.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps with high content of cordycepin and the culture medium used therein. Background technique [0002] In 1950, Cunningham et al in Germany observed that the insect tissue parasitized by Cordyceps militaris was not perishable, and then isolated an antibacterial substance from it, named it cordycepin, and published a paper in Nature. [0003] After a lot of research, cordycepin is a nucleotide composed of adenosine and deoxypentose with a carbon branch, so it is called 3'-deoxyadenosine (3'-deoxyade-nosine, 3'-dA) , was the first deoxynucleoside antibiotic isolated from a fungus. The molecular formula of cordycepin is C 10 h 13 N 5 o 3 , Molecular weight 251D, alkaline, needle or sheet crystal. The melting point is 230°C-231°C, and the maximum absorption wavelength of ultraviolet light is 259nm. Cordycepin is soluble in normal saline, hot ethanol and methanol, but insoluble in benzene, ether ...

AI Technical Summary

Problems solved by technology

[0007] At present, although there are many domestic and foreign companies that can produce Cordyceps militaris, because the key technologies such as industrialized large-scale cultivation have not yet broken through, the content of cordycepin in their products is very low, and the cost of obtaining cordycepin is very high, so the price of cordycepin is extremely high. High, up to 20,000-40,000 RMB per gram, making it difficult for ordinary people to use cordycepin