Cultivation method for potato breeder's seeds
A cultivation method and original seed technology, applied in the cultivation field of original potato seed, can solve problems such as easy formation of high-temperature and high-humidity microenvironment, small number of tubers per plant, unfavorable successor original seed production, etc., and achieve improvement of plant quality. The growth and the yield of the original original seed, the number of tubers on the plant and the size of the tuber block are improved, which is beneficial to the production of the subsequent original seed
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Embodiment 1
[0035] A kind of cultivation method of original seed of potato, the step comprises:
[0036] (1) Rapid propagation of virus-free seedlings: 30 strains / bottle of virus-free seedlings are used to inoculate the culture medium (rapid propagation medium is MS+3×10 -3 paclobutrazol) in culture flasks, cultured for 15 days at 25°C, 2000Lx, 16h / 8h photoperiod;
[0037] Among them, the stubble inoculation means leaving a stem section at the base of the mother seedling without cutting it, and continuing to replay it in the culture room for cultivation. When inoculating, the cut stem section does not need to be inserted on the medium, but can be evenly placed on the surface of the medium. The propagation medium requires softer hardness to facilitate rooting of stem segments and germination of axillary buds. No strong seedling cultivation is required before transplanting.
[0038] (2) Seedling hardening: transfer the tissue-cultured seedlings to a room with scattered light in the middle...
Embodiment 2
[0048] A kind of cultivation method of original seed of potato, the step comprises:
[0049] (1) Rapid propagation of virus-free seedlings: 35 strains / bottle of virus-free seedlings are used to inoculate into the culture medium (rapid propagation medium is MS+3×10 -3 paclobutrazol) in culture flasks, cultured for 20d at 25°C, 2000Lx, 16h / 8h photoperiod;
[0050] Among them, the stubble inoculation means leaving a stem section at the base of the mother seedling without cutting it, and continuing to replay it in the culture room for cultivation. When inoculating, the cut stem section does not need to be inserted on the medium, but can be evenly placed on the surface of the medium. The propagation medium requires softer hardness to facilitate rooting of stem segments and germination of axillary buds. No strong seedling cultivation is required before transplanting.
[0051] (2) Seedling hardening: transfer the tissue-cultured seedlings to a room with scattered light in the middl...
Embodiment 3
[0061] A kind of cultivation method of original seed of potato, the step comprises:
[0062] (1) Rapid propagation of virus-free seedlings: 40 strains / bottle of virus-free seedlings are used to inoculate into the culture medium (rapid propagation medium is MS+3×10 -3 paclobutrazol) in culture flasks, cultured for 25 days at 25°C, 2000Lx, and 16h / 8h photoperiod;
[0063] Among them, the stubble inoculation means leaving a stem section at the base of the mother seedling without cutting it, and continuing to replay it in the culture room for cultivation. When inoculating, the cut stem section does not need to be inserted on the medium, but can be evenly placed on the surface of the medium. The propagation medium requires softer hardness to facilitate rooting of stem segments and germination of axillary buds. No strong seedling cultivation is required before transplanting.
[0064] (2) Seedling hardening: transfer the tissue-cultured seedlings to a room with scattered light in t...
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