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Preparation method of agaricus bisporus liquid strain

A technology of liquid strains and Agaricus bisporus, which is applied in the field of preparation of liquid strains of Agaricus bisporus, can solve the problems of easy breeding of miscellaneous bacteria and unreasonable medium ratio, etc., and achieve the effects of enhancing the vitality of the strains, shortening the growth cycle, and improving the quality

Inactive Publication Date: 2016-11-23
山东省科创食用菌产业技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the culture process of Agaricus bisporus liquid strains, there are problems such as unreasonable proportion of various components of the medium and easy breeding of miscellaneous bacteria. Therefore, it is of great market value to develop a high-yield and high-quality liquid strain for producing Agaricus bisporus

Method used

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  • Preparation method of agaricus bisporus liquid strain

Examples

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Embodiment 1

[0028] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:

[0029] (1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 5 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2cm;

[0030] The slant culture medium is prepared by adding 8 mL of green tea extract, 25 g of cornstarch, 2 g of agar, 0.5 g of casein hydrolyzed peptide, 0.8 g of peptone, and 0.35 g of potassium dihydrogen phosphate per 1000 mL of purified water, stirring evenly, and cooling after sterilization ;

[0031] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture them on a shaking table at a temperature of 20-22°C for 2 days, and then culture them statically at 20-22°C for 1 day to obtain primary culture medium;

[0032] Add 20mL of green tea extract, 10g of peptone, 50g of ...

Embodiment 2

[0038] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:

[0039] (1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 6 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2 cm;

[0040] The slant culture medium is prepared by adding 9 mL of green tea extract, 20 g of cornstarch, 1 g of agar, 0.3 g of casein hydrolyzed peptide, 1.0 g of peptone, and 0.5 g of potassium dihydrogen phosphate for every 1000 mL of purified water, stirring evenly, and cooling after sterilization ;

[0041] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture it on a shaking table at a temperature of 20-22°C for 1 day, and then culture it statically at 20-22°C for 2 days to obtain primary culture medium;

[0042] 15mL of green tea extract, 9g of peptone, 53g of sol...

Embodiment 3

[0048] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:

[0049](1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 5 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2cm;

[0050] The slant culture medium is prepared by adding 10mL green tea extract, 28g cornstarch, 2g agar, 0.2g casein hydrolyzate peptide, 1.2g peptone, 0.2g potassium dihydrogen phosphate to every 1000mL purified water, stir evenly, and cool down after sterilization ;

[0051] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture them on a shaking table at a temperature of 20-22°C for 2 days, and then culture them statically at 20-22°C for 1 day to obtain primary culture medium;

[0052] Add 18mL of green tea extract, 7g of peptone, 55g of soluble starch, 8g of glycerol...

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Abstract

The invention belongs to the technical field of edible fungi strain preparation and relates to a preparation method of an agaricus bisporus liquid strain. The preparation method comprises propagation expanding culture using a slant culture medium, first-level and second-level culture media and a fermentation culture medium; the formulas of the culture mediums provided by the invention can provide the nutritional ingredients required in the growth process of the agaricus bisporus strain, the growth cycle of the agaricus bisporus liquid strain is shortened, and the strain activity is enhanced; according to the invention, by adding a little green tea extract, the generation of mould, sundry fungi and the like can be effectively prevented in the growth process of agaricus bisporus; and moreover, the growth of agaricus bisporus strain is promoted, the mycelia grow fast, the quality is good, and the purity is high.

Description

technical field [0001] The invention belongs to the technical field of preparation of edible fungus strains, and relates to a preparation method of Agaricus bisporus liquid strains. Background technique [0002] Agaricus bisporus, also known as white mushroom or mushroom, is an edible fungus with the largest cultivation area, the largest output, and the most common consumption in the world. Agaricus bisporus is white in color, soft in texture, rich in nutrition and delicious in taste. It is rich in protein, various amino acids, vitamins and minerals. Cholesterol and other health functions, is an ideal health food. [0003] With the increase in the price of sawdust, etc., the price of cultivating solid strains also rises, and the cultivation time of solid strains is long, and the growth rate of bacteria is slow. Due to the vigorous growth of mycelia, short bacterial age, mushroom gas, and fast tide change, the liquid strains whose quality and output are significantly higher...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 刘孝利李晓博李召义高霞
Owner 山东省科创食用菌产业技术研究院
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