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Application of ulinastatin in preparation of drug for treating gallbladder carcinomas

A technology for ulinastatin and gallbladder cancer, which is applied in the field of medicine, can solve the problem of gallbladder cancer not found yet, and achieve remarkable curative effect and low toxic and side effects

Inactive Publication Date: 2016-11-16
GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also studies that show that ulinastatin has a good inhibitory effect on cancer cells such as lung cancer, gastric cancer, liver cancer, breast cancer, and cervical cancer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, preparation ulinastatin freeze-dried powder injection

[0013] Take 200 million units of filtered sterilized ulinastatin aqueous solution, add 20 grams of mannitol to dissolve, adjust the pH to neutral, add water for injection to 2000 ml, add sodium chloride to adjust isotonicity, filter aseptically, and pack in 1000 pieces In a vial, freeze-dried under sterile conditions, to obtain.

Embodiment 2

[0014] Embodiment 2, preparation ulinastatin injection

[0015] Take 100 million units of filtered sterilized ulinastatin aqueous solution, add 20 grams of mannitol to dissolve, adjust the pH to neutral, add water for injection to 2000 ml, add sodium chloride to adjust isotonicity, filter aseptically, and pack 1000 pieces Get it in a vial.

Embodiment 3

[0016] Example 3. Study on the Effect of Ulinastatin on Transplanted Tumors of Human Gallbladder Cancer in Nude Mice

[0017] 1. Materials

[0018] Animals: 46 BALB / c-nu / nu female nude mice, aged 7-8 weeks, weighing 18-20g, provided by the Animal Experiment Center of Guangdong Medical College; cell line: human gallbladder cancer cell line GBC-SD, purchased from Institute of Cellular Science, China; cisplatin, purchased from Nanjing Pharmaceutical Factory Co., Ltd., approved by H20104016.

[0019] 2. Method

[0020] (1) Establishment of human gallbladder cancer xenograft tumor model in nude mice:

[0021] a. Cell resuscitation: Human gallbladder cancer cells frozen in liquid nitrogen were taken out and immediately placed in a 37°C water bath for 10-15 minutes to resuscitate.

[0022] b. Transfer the recovered cell suspension to a clean 15ml polypropylene alcohol centrifuge tube, add 10ml serum-free 1640 medium, mix gently, centrifuge at 4000rpm for 5 minutes and discard the ...

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Abstract

The invention belongs to the technical field of medicines, and particularly discloses application of ulinastatin in preparation of a drug for treating gallbladder carcinomas. It is proved through animal experiments that ulinastatin has an obvious inhibiting effect on human gallbladder carcinoma transplanted tumor in nude mice, is low in toxic and side effects and high in safety and can serve as a drug for treating the gallbladder carcinomas for use.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically discloses the use of ulinastatin in the preparation of medicines for treating gallbladder cancer. Background technique [0002] Gallbladder cancer (carcinoma of the gall-blader) occupies the first place among gallbladder malignant tumors, and other types include sarcoma, carcinoid, primary malignant melanoma, and giant cell adenocarcinoma. Primary gallbladder carcinoma is relatively rare clinically, and has not attracted people's attention for a long time. According to domestic textbook reports, it only accounts for about 1% of all cancers. Due to the extensive development of imaging examinations such as B-ultrasound and CT, gallbladder cancer has been gradually recognized and the detection rate has increased. However, the incidence of gallbladder cancer has obvious regional differences, and the incidence of gallbladder cancer patients is 2 to 4 times higher in women than in me...

Claims

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Application Information

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IPC IPC(8): A61K38/57A61P35/00
CPCA61K38/57
Inventor 孙明晖李翰明赵菁宋建东
Owner GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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