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Embedding for arbuscular mycorrhizal fungi agent and preparation of capsule fungi agent

A technology of arbuscular mycorrhizal fungi and capsule bacteria, which is applied in the field of embedding and preparation of capsule bacterial agents, can solve the problems of inability to carry out pure culture and affect the large-scale production of fungal agents, and achieve the goal of being easily absorbed by microorganisms. The effect of decomposition

Inactive Publication Date: 2016-11-16
TONGLING DONGSHENG ECOLOGICAL AGRI SCI & TECH CO LTD
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AI Technical Summary

Problems solved by technology

AM fungi are widely used, but so far cannot be purely cultured, which affects the large-scale production of fungal agents and the promotion of large-scale agriculture

Method used

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  • Embedding for arbuscular mycorrhizal fungi agent and preparation of capsule fungi agent

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Embodiment Construction

[0012] 1. The embedding of an arbuscular mycorrhizal fungal inoculum and the preparation of a capsule inoculum, the steps are as follows

[0013] 1), peat, cinder, malt root, rice are dried and ground into powder;

[0014] 2) G. intrarhizogi and Ri-DNA transformed carrot roots were cultured in an improved sterile synthetic medium. The whole culture was fully stirred with a magnetic stirrer, passed through a 400-mesh sieve, and washed with sterile water to remove The mixed inoculant containing infectious spores, hyphae and mycorrhizae can be collected by solid glue;

[0015] 3) With 2% sodium alginate as the basic carrier, add 2% chitosan, 3% gelatin, 5% glycerin, 3% peat, 1% coal cinder, 1% malt root, and 3% rice flour filler , 101kPa, 121°C high-pressure steam sterilization for 30 minutes, when the temperature is lower than 40°C, add liquid bacterial agent and mix evenly to form a homogeneous liquid flow, and drip the homogeneous liquid flow into 1.5%-4.5% through a constant...

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Abstract

The invention discloses an embedding method of an arbuscular mycorrhizal fungus and a preparation method of a capsule fungus. The invention uses sodium alginate as a basic carrier to spore, mycorrhizal and hyphae of the fungus Glomus intraroote Embedded in gel particles, the complete infection process can be completed when sown in non-sterilized soil, and the infection rate has a certain increase with the extension of the infection time. Chitosan, gelatin, glycerin, rice, peat, coal cinder, and malt root added different nutrients, so that the infection rate of the bacterial agent was different, and the infection rate of rice was the highest, reaching 42.9% after 25 days.

Description

technical field [0001] The invention relates to the embedding of an arbuscular mycorrhizal fungus inoculum and the preparation of a capsule inoculum. Background technique [0002] Arbuscular mycorrhizal fungi are endomycorrhizal fungi that exist in soil and can live in symbiosis with plants. It exists in the roots of a variety of plants, including food crops, vegetables, fruits, medicinal plants and some economic crops, etc. It is one of the microorganisms most closely related to plants discovered so far. The AM fungus and the host plant live in a mutualistic symbiosis. It absorbs nutrients such as nitrogen, phosphorus, potassium, calcium, magnesium and other trace mineral elements from the soil and transmits them to the host plant, and the host plant will supply the carbohydrates necessary for the growth of the AM fungus . AM fungi are widely used, but so far cannot be purely cultured, which affects the large-scale production of fungal agents and the promotion of agricult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 房振东
Owner TONGLING DONGSHENG ECOLOGICAL AGRI SCI & TECH CO LTD
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