Combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in blood sample of living body

Abstract

The invention provides a combined liquid chromatography-mass spectroscopy method for high-flux detection of 113 lipids in a blood sample of a living body, belonging to the field of bioanalytical chemistry. The method comprises the following steps: subjecting a to-be-detected sample to pretreatment; then adding a reaction solvent and an extraction agent for extraction; and then carrying out liquid chromatographic-mass spectroscopic detection. The method is simple to operate and high in selectivity and sensitivity, and can rapidly detect 113 lipids in blood, wherein the 113 lipids include 48 of phosphatidylcholines (PC), 21 lysophosphatidylcholines (Lyso-PC), 17 phosphatidylethanolamines (PE), 4 lysophosphatidylethanolamines (LPE), 6 lysophosphatidylinositols (PI), 9 sphingomyelins (SM) and 8 ceramides (Cer). The method has precision meeting requirements on analysis of serum samples, good stability and high detection sensitivity to most lipid metabolites, and is applicable to lipidomic analysis of serum samples.

Description

technical field [0001] The invention relates to a liquid mass spectrometry method for high-throughput detection of 113 kinds of lipids in biological blood samples, which belongs to the field of biochemical analysis and detection, and can quickly and high-throughput detect 113 kinds of lipid components in blood. The detection efficiency and accuracy are greatly improved. Background technique [0002] Blood is an important part of the human body. It flows through various organs and tissues in the human body, participates in the metabolism of the human body, regulates functions, and maintains the balance of the internal and external environment of the human body. When pathological changes occur in any part of the human body, specific biomarkers will be released into the blood. In addition, blood sample detection is non-invasive and is the most readily available biological sample that can be used as in vitro diagnosis, so blood routine is also the most direct and easy-to-obtain ...

AI Technical Summary

Problems solved by technology

[0006] The amount of lipids in the blood detected in the existing reports is small, or only a single type of lipids, and the detection results of different detection instruments used in different reports are not uniform, and most methods combine Metabolic correlation network analysis and database retrieval are used to analyze and deduce the structure of possible biomarkers, instead of using standard products for identification. The accuracy of the results is questionable and cannot provide true and effective detection standards.

There is no report on the simultaneous detection of seven types of 113 lipids using a pretreatment method followed by liquid chromatography tandem mass spectrometry and identification by all standards

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