Compound microorganism preparation for preventing and treating rice blast and preparing method of compound microorganism preparation
A technology of composite microorganisms and rice blast, which is applied in the field of composite microbial preparations and preparations for the prevention and treatment of rice blast, can solve the problems of single mode of action, unsatisfactory production and use, unstable control effect, etc., and achieve pertinence Strong, super strong control of rice blast, high efficacy
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Embodiment 1
[0030] A compound microbial preparation for preventing and treating rice blast. The raw material ratio of the microbial preparation is: 30 parts of fermentation broth of Streptomyces tsushima, 40 parts of fermentation broth of Streptomyces syringae, 30 parts of fermentation broth of Bacillus arborii, and brevibacillus molybdenum 30 parts of Bacillus fermentation liquid.
[0031] In order to achieve the above object, the preparation method of the compound microbial preparation for preventing and treating rice blast comprises the following steps:
[0032] Step 1: Preparation of Streptomyces tsushima fermentation broth
[0033] Take out the preservation tube of Streptomyces tsushima strain, use Gaoshi No. 1 solid medium to scratch the plate for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When the lawn is overgr...
Embodiment 2
[0052] Embodiment 2 plate antibacterial test
[0053] According to the plate confrontation culture test method in the "Plant Disease Research Method", the compound microbial preparation prepared in Example 1 is evaluated on the inhibitory effect of the rice blast bacterium, and when the clear water contrast treatment group is covered with the plate, the statistical results are as follows, the 44%。 Composite microbial preparations growth inhibition rate of blast fungus was 95.44%.
Embodiment 3
[0054] Example 3 Composite microbial preparation is tested in rice blast greenhouse
[0055] 6.7m per plot 2 , three repetitions; rice plants were inoculated with pathogenic bacteria when they reached the tillering stage;
[0056] After inoculating the spores of Magnaporthe grisea, spray 30ml of compound microbial preparation for the first time when the control group begins to morbidity, dilute it 100 times and spray, and then spray the bacterial preparation once every 7-10 days, three times in a row. Until the harvest of rice, investigate the control effect and yield; 20% tricyclazole WP (Jiangsu Fengdeng Agrochemical Co., Ltd.) was used as the control agent. The blank control group was sprayed with water each time.
[0057] Test results: As shown in Table 1, the control effect of the compound microbial preparation on rice blast was 90.74% in the greenhouse test, much higher than that of the tricyclazole treatment group. At the same time, the use of this composite microbia...
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