Cycloastragenol extract and preparation method and application thereof
A technology of cycloastragenol and extract, which is applied in the field of medicine, can solve the problems of very few studies on cycloastragenol extract, and achieve the effects of slowing down aging damage, improving cell vitality, and anti-aging activity
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Embodiment 1
[0033] Embodiment 1 The preparation method of the astragaloside IV crude extract of the present invention:
[0034]The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH of 13 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure, and then freeze-dry to obtain astragaloside IV The crude extract was 0.8084g, and the content of astragaloside IV was determined to be 0.7399% by HPLC detection.
Embodiment 2
[0035] Embodiment 2 The preparation method of astragaloside IV crude extract of the present invention:
[0036] The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH of 12 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure and then freeze-dry to obtain astragaloside IV The crude extract was 0.8080g, and the content of astragaloside IV was determined to be 0.7381% by HPLC detection.
Embodiment 3
[0037] Embodiment 3 The preparation method of the astragaloside IV crude extract of the present invention:
[0038] The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH 11 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure and then freeze-dry to obtain astragaloside IV The crude extract was 0.9088g, and the content of astragaloside IV was determined to be 0.6596% by HPLC detection.
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