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A method for preparing a microchannel array plate, a device for obtaining liquid droplets and a method for producing liquid droplets

An array plate, microchannel technology, applied in chemical instruments and methods, transportation and packaging, fluid controllers, etc., can solve problems such as difficulty in obtaining wider applications, clogging, and low success rate

Active Publication Date: 2019-05-24
因纽美瑞克斯公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These shortcomings make it difficult for the microfluidic devices commonly found in bioanalytical chemistry laboratories to be more widely used.
In response to the above problems, cn104741158A proposed a device and method for generating micro-droplets by using inertial force, but in this device, a capillary array is used as a droplet forming device, and the capillary array needs to be drawn, the success rate is low, and the thickness of the capillary is uneven , prone to clogging, poor experimental parallelism, and difficult to achieve high-throughput production

Method used

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  • A method for preparing a microchannel array plate, a device for obtaining liquid droplets and a method for producing liquid droplets
  • A method for preparing a microchannel array plate, a device for obtaining liquid droplets and a method for producing liquid droplets
  • A method for preparing a microchannel array plate, a device for obtaining liquid droplets and a method for producing liquid droplets

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] The cooperation of embodiment 1 microchannel plate and collection device

[0104] Microchannel plate with 1.5mL centrifuge tube

[0105] Add 1ml of the second liquid into the centrifuge tube. Because the second liquid is easy to generate air bubbles, it must be added slowly. After the air bubbles are formed, you can use a 1ml pipette to replace the tip with a new pipette, and quickly aim at the air bubbles to pump out the air. The air bubbles will disappear immediately. was blown apart.

[0106] Assembly diagram as Figure 4 shown. 1 bolt, 1.1 bolt head, 1.2 bolt lower surface, 1.3 male thread, 1.4 through hole, 2 spacer, 3 microchannel array plate, 4 connection part, 4.1 connection part head, 4.2 connection part lower end surface, 4.3 female thread, 4.4 Inner end, 5 1.5mL centrifuge tubes (the caps are omitted here). After the two threads are tightened, fix the microchannel plate and place it lightly in the centrifuge tube filled with the second liquid.

[0107] M...

Embodiment 2

[0111] The droplets produced by the device of the present invention are used in digital chain reaction (dPCR) detection of trace DNA samples based on TaqMan probes

[0112] The second liquid formulation in this example was isopropyl laurate / Abilem 180 v / v 83 / 17 solution and the collection device was a 1.5 ml centrifuge tube.

[0113] The preparation method of the first liquid

[0114] First prepare the following mixture (1), wherein the primers are designed based on a 223bp sequence in lambda phage DNA for PCR amplification, and TaqMan probes are also designed based on this sequence.

[0115]

[0116]After obtaining the mixed solution (1), take 99 μl of the mixed solution and add 1 μl of DNA template. This DNA template is the product after agarose gel purification, and its concentration is determined by Nanodrop. Add 1 μl of 1.00*10^6 copy and 1 μl of 1.00*10^5 to 99 μl of the mixture (1) respectively to obtain reaction samples A and B. in

[0117]

A

B

...

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Abstract

The invention relates to a preparation method of a microchannel array plate, comprising the following steps: (1) taking two different optical fiber glass rods, one of which cannot be corroded by corrosive liquid, and one of which can be corroded by corrosive liquid, and the two are closely connected Arranged, melted into a whole at high temperature, drawn into long and thin glass rods one or more times, and cut the thin glass rods into small pieces to obtain microchannel plate blanks; (2) Etching and removing the blanks Core material to obtain a microchannel plate with through holes; (3) carry out hydrophobic treatment to the microchannel plate. Using the microchannel array plate of the present invention in cooperation with the collecting device and the acceleration generating device can rapidly generate uniform micron-sized liquid droplets with high throughput by using inertial force.

Description

technical field [0001] The invention relates to a preparation method of a microchannel array plate, a device for obtaining liquid droplets and a method for producing liquid droplets. Background technique [0002] Emulsion droplet technology is a crucial part of many molecular biology experiments. Uniform, stable, and compatible liquid droplets for biological experiments have been demonstrated in many technologies and applications. Among them, technologies and methods such as cell culture, sample separation, digital polymerase chain reaction and emulsion whole genome amplification are common. Droplet technology is likely to become the technical cornerstone of next-generation sequencing, third-generation PCR reactions, and related high-throughput biological tests. On the one hand, many isolated and independent solution environments caused by droplets can form many tiny reaction vessels, which greatly reduces the amount of samples; Excellent choice for molecular amplificatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/0241B01L2200/12B01L2200/061B01L2300/0861B01L2300/0829B01L2400/0409B01L2300/165B01L2200/0673B01L2300/0832B01L2300/161B01F23/4145B01F23/41B01F25/20B01F35/71725
Inventor 黄岩谊陈子天廖珮宇张芳丽
Owner 因纽美瑞克斯公司
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