Anti-acne snake venom compound external application preparation and preparation method thereof
A technology of topical agent and snake venom, which is applied in the field of anti-acne snake venom compound topical agent and its preparation, which can solve the problems of skin penetration, absorption stability, low targeting, skin irritation and drug resistance, and insufficient clinical curative effect Ideal and other issues, to achieve the effect of reducing skin irritation and drug resistance, reducing sebum secretion, and avoiding skin adverse reactions
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[0020] Dissolve 1g of cobra snake venom in 100ml of deionized water, centrifuge at 4°C and 5000rpm for 15 minutes, and take the supernatant for gel filtration chromatography; gel filtration uses Sephadex-G-75 gel column with a column height of 100cm and an inner diameter of 1.6cm , the eluent is the ammonium acetate solution of 0.05mol / L, the elution rate is 16ml / h, and 1 tube is collected in 10 minutes; the toxin components that cause hemorrhage are removed after separation, and the remaining components are freeze-dried to obtain the refined cobra venom dry powder.
[0021] Baicalein is extracted from Scutellaria baicalensis Chinese medicinal materials, the specific implementation method:
[0022] Add 100g of medicinal material Scutellaria baicalensis, add 500ml of distilled water after crushing, pass through a 20-mesh sieve after fully stirring, add 2000U cellulase at 40°C, pH4.5 for enzymolysis for 24 hours, then suction filter, add 60% ethanol to the solid obtained by suct...
Embodiment 1
[0024] Embodiment 1, containing cobra family snake venom dry powder 70g, baicalein product 30g in every 100g mixture;
Embodiment 2
[0025] Embodiment 2, containing cobra family snake venom dry powder 65g, baicalein product 35g in every 100g mixture;
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