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Sterile cultivation device capable of achieving automatic monitoring and regulation of multiple culture conditions

A technology of culture conditions and automatic monitoring, applied in the field of plant tissue culture, can solve the problems of bacteria contamination and unstable external environment, and achieve the effect of simple and convenient operation and automation.

Active Publication Date: 2016-11-09
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional tissue culture operation requires frequent replacement of the culture medium. During the replacement of the culture medium, there is a possibility of bacterial contamination. Stable and sterile, but these external environments are often unstable during culture

Method used

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  • Sterile cultivation device capable of achieving automatic monitoring and regulation of multiple culture conditions
  • Sterile cultivation device capable of achieving automatic monitoring and regulation of multiple culture conditions

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Embodiment Construction

[0012] Below in conjunction with accompanying drawing and embodiment the present invention will be further described: as figure 1 and 2 as shown,

[0013] The aseptic cultivation device capable of automatically monitoring and regulating a plurality of cultivation conditions of the present invention comprises: a water inlet 1, a water outlet 2, a water storage tank 3, a waste water tank 4, a CPU microprocessor 5, and an aqueduct interface 6 with a bacteria-filtering film , multi-interface suction pump 7, water guide pipe interface 8, oxygen / carbon dioxide concentration meter 9, temperature sensitive sensor 10, pH meter 11, pressure sensor 12, ion concentration detector 13, humidity sensor 14, clapboard 15, seedling raising sponge 16, Cultivation device main body 17, cultivation device cover 18, sensor line channel 19, water guide channel 20 and water injection channel 21, water inlet 1 and water outlet 2 are respectively located on water storage tank 3 and waste water tank 4, ...

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Abstract

The invention relates to a sterile cultivation device capable of achieving automatic monitoring and regulation of multiple culture conditions. The device mainly comprises a cultivation device body, a water inlet, a water outlet, a water storage tank, a wastewater tank, a belt filter pellicle water manifold interface, a multi-interface water suction pump, a water manifold interface, a CPU microprocessor, an oxygen / carbon dioxide monitor, a thermosensitive sensor and other detection instruments, a PH instrument, a pressure sensor, an ion concentration detector and a moisture sensor, wherein the oxygen / carbon dioxide monitor, the thermosensitive sensor, the PH instrument, the pressure sensor, the ion concentration detector and the moisture sensor are connected with the CPU microprocessor through sensor line channels respectively, the water storage tank and the wastewater tank are connected with the cultivation device body into a whole and communicated with a water guiding hose through the multi-interface water suction pump, and the water guiding hose is connected with a filtering and degerming unit; a sterile environment in the cultivation device is guaranteed, whether a plant growth environment is normal is monitored continuously, and normal PH of culture solution in the water storage tank is guaranteed. By the adoption of the device, automatic cultivation can be achieved, long-term cultivation of aseptic seedlings is achieved, and operation is easy and convenient.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a cultivation device, in particular to an aseptic cultivation device capable of automatically monitoring and regulating multiple cultivation conditions. Background technique [0002] Plant tissue culture is different from conventional tissue culture techniques. Test-tube plantlets need to be replaced frequently with new medium, and most of test-tube plantlets are rootless seedlings, which can only be transplanted after rooting. The rooting solid medium of test-tube plantlets is troubled by various factors such as medium components, phytohormones, and light, and test-tube plantlets need to survive in a sterile state for a long time no matter whether they are to preserve the seeds or continue to the next experiment or production. time. Therefore, the cultivation environment should be sealed, the moisture in the medium should be maintained, the growth of test tube se...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 冯宝民靳巧春于宗霞卢轩秦海宏王惠国
Owner DALIAN UNIV
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