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3D cell culture system

A cell culture, 3D technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve problems such as adverse effects, time-consuming and laborious, cell contamination, etc., to save labor costs, unmanned Chemical operation, avoid the effect of contamination

Inactive Publication Date: 2016-10-26
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional artificial cell culture is not only time-consuming and labor-intensive, errors often occur, and even leads to cell contamination, but also artificial detection of the cell culture environment cannot achieve real-time analysis and control, which has adverse effects on cell experiments or industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this example, see figure 1 , the 3D cell culture system includes a cell culture dish (1), a sterile hose (2), a peristaltic pump (3), an optical fiber sensor (4), a signal processing system (5), a temperature controller (6), Gas controller (7), cell culture solution storage tank (8), carbon dioxide gas storage tank (9), computer and supporting control software (10) and light source (11). It is characterized in that two sterile hoses (2) are connected to the bottom of the cell culture dish (1), a temperature controller (6) is inserted and an optical fiber sensor (4) is connected, and the two sterile hoses ( 2) Through the peristaltic pump (3) and the gas controller (7), connect the cell culture solution storage tank (8) and the carbon dioxide gas storage tank (9) to deliver the cell culture solution and gas to the cell culture dish (1). carbon dioxide gas; one end of the optical fiber sensor (4) is connected to the light source (11), and the other end is connected to...

Embodiment 2

[0032] This embodiment is basically the same as Embodiment 1, especially in that:

[0033] The structure of the cell culture dish (1) is a general-purpose petri dish for cell experiments or a cell culture plate composed of multiple petri dishes. There are several interfaces at the bottom, and a bundle of optical fiber sensors (4) are used to detect the Temperature, pH value and refractive index; two sterile hoses (2) are connected to add culture medium and carbon dioxide to the cell culture dish through peristaltic pump (3) and gas controller (7); add temperature control The device (6) can regulate the internal temperature of the culture medium.

[0034] The structure of the optical fiber sensor (4) is divided into two optical fiber transmission sections and a sensing section. One of the optical fiber transmission sections receives the injection of standard laser light from the light source (11). When the standard laser light is transmitted to the sensing section, it is affec...

Embodiment 3

[0037] In this example, the system can be combined with a multi-well cell culture plate ( figure 2 , image 3 ), also known as a multi-dish system, each culture well or dish is independently installed with the same optical fiber sensor (4), sterile soft (2) tube, temperature controller (6) and other supporting equipment as in the embodiment , and reasonably arrange the lines of optical fibers, hoses and wires on the culture plate ( image 3 ). All culture dishes in the whole system can work simultaneously and independently, and each well or dish can achieve different culture conditions, which are individually controlled by the computer to meet the needs of different cell experiments.

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Abstract

The invention discloses a 3D three-dimensional cell culture system which is applicable to cell experiments and bio-engineering production. The system comprises a cell culture dish, sterile soft hoses, a peristaltic pump, an optical fiber sensor, a signal processing system, a temperature controller, a gas controller, a cell culture solution storage tank, a carbon dioxide storage tank, a computer as well as associated software and a light source. According to a working principle, such information as temperature, pH, cell density and the like in the cell culture dish is monitored by virtue of the optical fiber sensor in real time and the information is analyzed and processed by virtue of the computer, so that related components are controlled to precisely regulate corresponding culture conditions, and various indexes in a cell culture medium are kept balanced and stable. The system can automatically complete such complex experimental operations as cell observation, culture solution replacing, temperature measurement, pH value regulation and the like, manual labor can be replaced, so that manual cost is reduced; and meanwhile, the system, which precisely controls culture conditions, can guarantee the stability and uniformity of experimental factors.

Description

technical field [0001] The invention relates to a 3D cell culture system that can be applied in cell experiments and bioengineering production, which can automatically maintain stable cell culture conditions through sensors and various control components, and has automation, intelligence, unattended and real-time monitoring The application can reduce labor cost and improve experiment efficiency, and belongs to the technical field of biological experiment equipment. Background technique [0002] Cell culture is to take out the tissue (or organ) in the animal body by aseptic operation, simulate the physiological conditions in the animal body, and cultivate it in vitro to make it grow and reproduce continuously. People use it to observe the growth, reproduction, and Differentiation and cellular senescence and other processes of life. The outstanding advantages of cell culture are: first, it is convenient to study the influence of various physical, chemical and other external f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/22C12M1/04
CPCC12M23/10C12M29/00C12M31/02C12M31/08C12M41/12C12M41/26C12M41/32C12M41/36C12M41/48
Inventor 刘书朋张恒童恺琛梁江
Owner SHANGHAI UNIV
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