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Improved Celsior preservation solution for donor heart, and preparation method and application thereof

A technology of preservation solution and double-distilled water, which is applied in the preservation and application of human or animal bodies, and medical preparations containing active ingredients, etc., which can solve the problems of detaching from the protective effect of the human body and unclear mechanism

Inactive Publication Date: 2016-10-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, linagliptin can only be used for clinical medical purposes, and its protective effect and mechanism in studies or experiments out of the human body are still unclear

Method used

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  • Improved Celsior preservation solution for donor heart, and preparation method and application thereof
  • Improved Celsior preservation solution for donor heart, and preparation method and application thereof
  • Improved Celsior preservation solution for donor heart, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, improving the formulation of Celsior donor heart preservation solution (taking 1000ml as an example).

[0029] Preparation of raw materials: potassium chloride 1.12g; magnesium chloride 2.64g; calcium chloride 0.01g; mannitol 10.93g; histidine 4.66g; sodium glutamate 3.74g; sodium lactobionate 6.90ml; reduced glutathione 0.92g; Linagliptin 1.18×10 -7 ~3.54×10 -7 g; enough double distilled water.

Embodiment 2

[0030] Example 2, the preparation method of the improved Celsior donor heart preservation solution (take the preparation of 1000ml as an example).

[0031] Add 800ml of double-distilled water into a container larger than 1000ml, then add 1.12g of potassium chloride, 2.64g of magnesium chloride, and 0.01g of calcium chloride in sequence, and stir until the color becomes clear; then add 10.93g of mannitol, 4.66g of histidine, gluten Sodium glutathione 3.74g, sodium lactobionate 6.90ml, stir until the color becomes clear; then add reduced glutathione 0.92g, linagliptin 1.18×10 -7 ~3.54×10 -7 g, stir until the color becomes clear; adjust the pH of the solution to 7.30, adjust the osmotic pressure to 320 mOsm / L, distill the volume to 1000ml with double distilled water, and store at 4°C at low temperature.

Embodiment 3

[0032] Example 3, the experiment of improving the effect of Celsior donor heart preservation solution.

[0033] Using the non-circulatory Langendorff perfusion function test model of the isolated mouse heart, compared with the simple Celsior donor heart preservation solution, a self-designed improved Celsior donor heart preservation solution was studied for a long time (9 hours) at 4°C for low temperature storage in vitro The preservation effect of the mouse heart.

[0034] Subjects: Male SD rats, weighing 250~300g.

[0035] According to the different components of the preservation solution, they are divided into:

[0036] Group 1: Celsior solution alone for asystole and preservation for 9 hours (Celsior);

[0037] Group 2: DPP-4 inhibitor low concentration (0.25nmol / L linagliptin in Celsior solution) and stored for 9 hours (0.25nM);

[0038] Group 3: Concentration of DPP-4 inhibitor (0.5nmol / L linagliptin in Celsior solution) and stored for 9 hours (0.5nM);

[0039] Group...

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Abstract

The invention discloses an improved Celsior preservation solution for a donor heart, and a preparation method and application thereof. The improved Celsior preservation solution for the donor heart comprises potassium chloride, magnesium chloride, calcium chloride, mannitol, histidine, reduced glutathione, monosodium glutamate, sodium lactobionate and linagliptin, with the balance being double-distilled water. The preparation method comprises the following steps: adding the double-distilled water into a container, then successively adding potassium chloride, magnesium chloride and calcium chloride, and carrying out stirring until the color of the obtained mixture becomes clear; then adding mannitol, histidine, reduced glutathione and monosodium glutamate and carrying out stirring until the color of the obtained mixture becomes clear; adding sodium lactobionate and carrying out stirring until the color of the obtained mixture becomes clear; and adjusting the pH value and osmotic pressure value of the obtained solution, fixing the volume of the solution with the double-distilled water and preserving the solution at a low temperature of 4 DEG C. The improved Celsior preservation solution has the advantages of good and long-term organ preservation effect and capacity of promoting recovery of heart functions after reperfusion of the donor heart.

Description

technical field [0001] The invention relates to the field of organ preservation solution, in particular to an improved Celsior donor heart preservation solution and a preparation method thereof. Background technique [0002] Heart transplantation is widely accepted as an effective treatment for end-stage cardiac disease. At present, cryopreservation is the most important measure in the process of clinically transporting donated hearts, but the effective preservation time is less than 6 hours, which makes it difficult to obtain heart donors remotely, and the recovery of cardiac function after reperfusion of donated hearts after cryopreservation Also inefficient. Therefore, it is the key to the success of heart transplantation to explore an effective preservation solution to prolong the time of simple low temperature storage, reduce the reperfusion injury of the donor heart, and promote the recovery of the heart function after long-term ischemia. [0003] Celsior solution is...

Claims

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Application Information

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IPC IPC(8): A01N1/02A61K31/522A61P9/10
CPCA01N1/0226A61K31/522
Inventor 陈莹莹郑鸣之何卉蕙沈晨露章逸洲沈岳良
Owner ZHEJIANG UNIV
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