Immunochromatography kit for testing of stomach function and preparation method of immunochromatography kit

Abstract

The invention relates to the technical field of immunochromatography detection and particularly discloses a kit for testing of the stomach function and a preparation method of the kit. The kit comprises a plastic board, a glass cellulose membrane, a nitrocellulose membrane, a sample pad and a water absorbent pad, wherein the glass cellulose membrane is coated with a quantum-dot-labeled mixture of pepsinogen I antibodies, pepsinogen II antibodies and helicobacter pylori urease antigens; a detection line 1, a detection line 2, a detection line 3 and a control line are arranged on the nitrocellulose membrane, the detection line 1 is coated with pepsinogen I secondary antibodies, the detection line 2 is coated with pepsinogen II secondary antibodies, the detection line 3 is coated with helicobacter pylori urease antigens, and the control line is coated with goat-anti-mouse lgG. According to the kit, fast and sensitive combined detection of pepsinogen I, pepsinogen II and helicobacter pylori urease antibodies is realized with the fluorescence immunochromatography method by means of the double-antibody / antigen sandwich principle in combination with the fluorescence characteristic of quantum dots.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography kit for gastric function detection and a preparation method thereof. Background technique [0002] Pepsin The inactive precursor of pepsin in the original gastric juice is secreted by the principal cells and mucus cells of the gastric body, most of which are secreted into the gastric cavity, and a small amount can be detected in the blood. According to its biochemical properties and immunogenicity, it is divided into 2 subgroups. Components 1-5 have the same immunogenicity and are called pepsinogen I, which is mainly secreted by the principal cells and mucous neck cells of the fundic gland; 6 and 7 are called pepsinogen II, in addition to being secreted by the principal cells and mucous neck cells of the fundic glands, the mucus neck cells of the pyloric glands of the cardia and antrum, and the upper duodenum can also produce pepsino...

AI Technical Summary

Problems solved by technology

Enzyme-linked immunosorbent assay is accurate in detection, can be accurately quantified, and has high sensitivity. However, the detection of samples requires steps such as sample addition, warm bath, washing, color development, and termination. The operating procedures are cumbersome and time-consuming. It takes at least 40 minutes and is not suitable for large-scale physical examination screening; sample detection requires a plate washer and microplate reader, which is inconvenient to carry, so it cannot be tested at the patient's home or in an ambulance; in addition, its sensitivity and specificity are not high enough , the accuracy of detection of low concentration samples is not high

However, chemiluminescence immunoassay, although it has high precision and fast detection speed, requires expensive chemiluminescence immunoassay analyzers and specific analysis rooms, and the cost of reagents is relatively high, so it cannot be widely used in grass-roots willingness and medical treatment. In the organization

Compared with enzyme-linked immunosorbent assay and chemiluminescence immunoassay, fluorescence immunochromatography has high detection sensitivity, high accuracy, simple operation and low cost, so it has more advantages in the detection of pepsinogen and Helicobacter pylori urease antibody. However, no matter what detection method is used at present, most of them are for the independent detection of pepsinogen I, pepsinogen II and Helicobacter pylori urease antibody, and the detection of pepsinogen I, pepsinogen One-step joint detection of three substances of II and Helicobacter pylori urease antibody

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