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Method for separating, purifying and identifying bacteria with super selenium endurance

A bacterial, selenium-resistant technology, applied in the directions of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., to achieve the effects of simple experimental methods, no environmental pollution, and low material costs

Inactive Publication Date: 2016-10-12
ANHUI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about the toxicity or impact of selenium compounds on Lysinibacillus (Lysinibacillus)

Method used

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  • Method for separating, purifying and identifying bacteria with super selenium endurance
  • Method for separating, purifying and identifying bacteria with super selenium endurance
  • Method for separating, purifying and identifying bacteria with super selenium endurance

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Embodiment Construction

[0036] In the following, the present invention will be further described in detail by describing the embodiments with reference to the accompanying drawings, so as to help those skilled in the art have a more complete, accurate and in-depth understanding of the inventive concepts and technical solutions of the present invention.

[0037] A method for the isolation, purification and identification of super selenium-resistant bacteria, the specific steps comprising:

[0038] (1) Preparation of soil sample suspension

[0039] Weigh 2g of selenium-enriched soil sample, put it into a conical flask with 50mL of sterilized phosphate buffer, and place it in a shaking incubator for shaking. The temperature of the shaking incubator is 25°C, and the speed is 180r min -1 , the shaking time is 30min, then stand still for 30min, take 30mL suspension in a sterilized 50mL centrifuge tube, place it in a centrifuge for centrifugation, and the speed of the centrifuge is 5000r·min -1 , centrifug...

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Abstract

The invention discloses a method for separating, purifying and identifying bacteria with super selenium endurance. The method includes the following steps of preparing selenium-rich soil suspension, carrying out enrichment culture, carrying out subculture, diluting a spread plate, carrying out plate streaking, activating, gram staining, total DNA extracting and 16S rDNA gene amplification for identification. The bacteria which have super selenium endurance and live in a selenium-rich environment are adopted, and the method has the advantages that selenium endurance is superhigh, material cost is low, an experiment method is simple, and environment pollution is avoided.

Description

technical field [0001] The invention belongs to the technical field of selenium-resistant microorganisms, and in particular relates to a method for separating, purifying and identifying super-selenium-tolerant bacteria. Background technique [0002] Selenium is an essential trace element for many organisms. In organisms, selenium is synthesized into selenoproteins. Some selenoproteins have anti-oxidation functions, and at the same time, can prevent and treat some cancers. When the content of selenium is low, it is beneficial to organisms, and when the content is high, it is harmful to humans and animals. Among the essential trace elements for the human body, selenium has the narrowest range from deficiency to toxicity. [0003] The currently reported selenium-tolerant microorganisms include Stenotrophomonas maltophilia, Bacillus, Rollestonella, Tauerella, Desulfuria, Hyperthermophilic Archaea, Rhizobium, Pseudomonas, etc. However, tolerance to selenium is not very high. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/36C12Q1/68C12Q1/04C12R1/01
CPCC12N1/20C12N1/02C12N1/36C12Q1/04C12Q1/689
Inventor 杨如意李静邵宗圆苏楠楠
Owner ANHUI NORMAL UNIV
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