Sea squirt-associated Penicillium citrinum Asc2-4

A technology of Penicillium citrinum and co-attachment, applied in the field of microorganisms, can solve problems such as poor stability, high cost, and complicated production process, and achieve the effects of tenacious vitality, fast growth speed, and simple process

Active Publication Date: 2016-10-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, commercially available polyunsaturated fatty acids, especially ω-3 polyunsaturated fatty acids, are mainly extracted from deep-sea fish oil. Higher plants and animals rarely contain ω-3 polyunsaturated fatty acids. The production process is complicated, the cost is high, and the stability is poor. are also limited by climatic conditions and resources

Method used

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  • Sea squirt-associated Penicillium citrinum Asc2-4
  • Sea squirt-associated Penicillium citrinum Asc2-4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Penicillium citrinum Asc2-4 separation and identification of

[0059] 1. Strain isolation

[0060] (1) Sample: sea squirt, collected from quicksand shrimp pond in Leizhou City, Guangdong Province.

[0061] Take 10g of the viscera of sea squirts, homogenize them with a homogenizer, put them into 90 mL of sterile seawater, shake them well and mix them evenly. Then use sterile seawater to press 10 -2 ~10 -6 Dilute and spread on solid medium, culture at 28°C for 24-72 hours, and isolate single colonies.

[0062] The above-mentioned single colony was further inoculated into a liquid seed medium, cultured on a shaker at 28°C for 72 hours, and the fat particles in the mycelia were observed under a microscope. Suction filter and dry the fermented mycelium, extract the oil in the mycelium by acid-heat method, measure the content of gamma-linolenic acid in the oil with a gas chromatograph, and select the strain with the highest gamma-linolenic acid content...

Embodiment 2

[0073] Example 2 Penicillium citrinum Asc2-4 Fermentation Preparation¶ - Linolenic oil

[0074] 1. A method for preparing gamma-linolenic acid oil by fermenting the ascidian symbiotic fungus Penicillium citrinum Asc2-4, comprising the following steps:

[0075] (1) Strain activation (solid strain culture):

[0076] Transfer the strains preserved in freeze-dried tubes or sand tubes to potato dextrose agar slant medium, and culture them at 25°C for 5 days to activate the strains;

[0077] (2) Seed liquid preparation (liquid strain culture):

[0078] The slant strains were made into spore suspension and inoculated in the seed medium for cultivation. The seed medium was potato dextrose medium, the inoculation amount was 3%, the pH was 5.5-6.5, the cultivation temperature was 28°C, and the cultivation time was 2 days ;

[0079] (3) Fermentation culture

[0080] 1) Fermentation medium preparation: Accurately weigh potatoes according to the proportion of 200-250g pota...

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Abstract

The invention discloses sea squirt-associated Penicillium citrinum Asc2-4. The sea squirt-associated Penicillium citrinum Asc2-4 is preserved in the Guangdong microbiology culture center on July 16, 2016 and has an accession number GDMCC NO: 60059. The sea squirt-associated Penicillium citrinum Asc2-4 is high gamma-linolenic acid yield filamentous fungus which is separated from sea squirt internal organs and is purified based on a potato extract glucose medium. The sea squirt-associated Penicillium citrinum Asc2-4 has a fast growth speed and strong vitality. The grease prepared through the sea squirt-associated Penicillium citrinum Asc2-4 fermentation has high gamma-linolenic acid content. The sea squirt-associated Penicillium citrinum Asc2-4 has a good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a strain of Ascidian symbiotic Penicillium citrinum Asc2-4. Background technique [0002] Sea squirt is a kind of marine organism, which belongs to chordate, urochordate, and sea squirt. It grows on pearl shell cages in natural sea areas. Studies have shown that marine invertebrate symbiotic microorganisms average hundreds of species per animal, and those with high density account for 40% of the total weight of the host animal, and most of them belong to the category of microorganisms that are difficult to cultivate. Sea squirts are animals that live in sedentary bodies. The outside of the sea squirt is sheathed by a layer of vesicles similar to plant cellulose, which is unique in the animal kingdom. The unique feeding and filtering system of sea squirts enriches a large number of microorganisms in the body and surface of the sea squirt. Compared with te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/80
CPCC12N1/14C12N1/145C12R2001/80
Inventor 雷晓凌钟敏聂芳红柴慧子刘唤明
Owner GUANGDONG OCEAN UNIVERSITY
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