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Frozen stock solution of immune cells

A technology of immune cells and cryopreservation solution, which is applied in the field of cell culture, can solve the problems of patients with physical disadvantages, immune rejection, infectious diseases, etc., and achieve the effect of reducing cytotoxicity, less cell damage, and protecting cells

Inactive Publication Date: 2016-10-12
GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

10% DMSO and 90% fetal bovine serum are mostly used in the existing cell cryopreservation solution. However, due to the high content of DMSO, it is quite toxic and unfavorable to patients, and fetal bovine serum contains a large amount of foreign proteins. There is a risk of infectious diseases, and it is easy to cause allergic reactions or immune rejection
Moreover, the survival rate and cell viability of this kind of cryopreservation solution are low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An immune cell cryopreservation solution, including the following components: recombinant human interleukin-2 50 U / mL, α-1,4-glucan 1.5 mg / mL, trehalose 0.1 mg / mL, polyethylene glycol 0.25 mL / mL, propylene glycol 0.05 mL / mL and RPMI-1640 medium 0.85 mL / mL.

Embodiment 2

[0019] An immune cell cryopreservation solution, including the following components: recombinant human interleukin-2 25 U / mL, α-1,4-glucan 1.0 mg / mL, trehalose 0.05 mg / mL, polyethylene glycol 0.3 mL / mL, propylene glycol 0.1 mL / mL and GT-T551 medium 0.9 mL / mL.

Embodiment 3

[0021] An immune cell cryopreservation solution, including the following components: recombinant human interleukin-2 100 U / mL, α-1,4-glucan 2.0 mg / mL, trehalose 0.15 mg / mL, polyethylene glycol 0.25 mL / mL, propylene glycol 0.15 mL / mL and GT-T561 medium 0.9 mL / mL.

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PUM

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Abstract

The invention discloses a frozen stock solution of immune cells. The frozen stock solution of the immune cells comprises the following components: 25 to 100 U / mL of recombinant human interleukin-2, 0.5 to 2.5 mg / mL of alpha-1,4-glucan, 0.05 to 0.2 mg / mL of trehalose, 0.1 to 0.5 mL / mL of polyethylene glycol, 0.05 to 0.15 mL / mL of propylene glycol and 0.7 to 0.9 mL / mL of a basic medium. The frozen stock solution of the immune cells disclosed by the invention has the beneficial effects of being high in safety, small in damage to the cells and capable of guaranteeing normal biological functions after the cells are recovered.

Description

technical field [0001] The invention belongs to the technical field of cell culture, in particular to an immune cell cryopreservation solution. Background technique [0002] Immune cell therapy is a new type of autoimmune anti-cancer treatment. It uses biotechnology and biological agents to culture and amplify immune cells collected from patients in vitro and then infuse them back into patients to stimulate and enhance immunity. Improve the immune function of the body, so as to achieve the purpose of treating tumors. Cell cryopreservation is one of the main methods for long-term preservation of cells. However, the process of cell cryopreservation will change the thermodynamic, chemical and physical environment of cells, and cause a certain degree of biological damage to cells. In order to minimize cell damage during cryopreservation and recovery, it is not only necessary to optimize the chemical and temperature manipulation process, but also to add one or more cryoprotectan...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 陈祥阮雯莉
Owner GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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