Carrot juice beverage and preparation method thereof
A carrot juice and carrot technology, which is applied in the field of carrot juice beverage and its preparation, can solve the problems of high fermentation conditions, vitamins are easily damaged by heat, increase production cost and the like, and achieve the advantages of simple preparation process, strong palatability and energy saving. Effect
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[0027] (1) Preparation of culture medium
[0028] MRS medium: peptone 10g / L, yeast extract 5g / L, beef extract 10g / L Tween 801ml / L, glucose 20g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, sodium acetate 5g / L, citric acid diamine 2g / L, pH6.2~6.6, sterilized at 115℃ for 30min.
[0029] (2) Preparation of starter
[0030] The Lactobacillus plantarum strain (purchased from Danisco (China) Co., Ltd.) was inserted into the MRS liquid medium, and it was activated by static cultivation at 30°C to 40°C for 12h to 24h. The inoculum was placed in MRS medium, cultured at 30°C to 40°C for 24h to 48h, centrifuged to collect the cells, washed with sterile saline for 2 to 3 times, and prepared to a concentration of 1 × 10 8 cfu / mL~1×10 9 cfu / mL of starter.
Embodiment 1
[0032] (1) Preparation of carrot juice: take commercially available concentrated carrot juice, add an appropriate amount of drinking water to adjust the soluble solid content to 10%, add 3% (mass percentage) of white sugar, and adjust the pH to 5.5 with baking soda , sterilize at 105℃ for 15min, cool to 35℃~40℃ naturally, for use;
[0033] (2) Fermentation: in the prepared carrot juice, take the carrot juice volume percentage as the inoculum of 0.5%, add the starter, stir well, ferment at 37°C for 36 hours, and measure the number of viable bacteria in the carrot juice to be 4.2 ×10 9 cfu / mL, pH 3.8, and carotenoid content above 50 mg / kg.
[0034] (3) Filling: Fill the fermented carrot juice with aseptic equipment and refrigerate at 0℃~4℃.
Embodiment 2
[0036] (1) Preparation of carrot juice: take commercially available concentrated carrot juice, add an appropriate amount of drinking water to adjust the soluble solid content to 15%, add 5% (mass percentage) of white sugar, and adjust the pH to 6.0 with baking soda , sterilize at 100℃ for 20min, cool to 35℃~40℃ naturally, set aside;
[0037] (2) Fermentation: in the prepared carrot juice, the inoculum volume percentage of carrot juice is 0.8%, the starter is added and the mixture is stirred evenly, and it is fermented at 37°C for 30 hours, and the number of viable bacteria in the carrot juice is measured as 3.7 ×10 9 cfu / mL, pH 4.2, and carotenoid content higher than 75 mg / kg.
[0038] (3) Filling: Fill the fermented carrot juice with aseptic equipment and refrigerate at 0℃~4℃.
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