Antimicrobial substrates and methods of use thereof

A substrate, alkyl technology, applied in the field of antibacterial substrates and their use, which can solve problems such as unmet needs

Inactive Publication Date: 2016-09-28
XMICROBIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Despite the above proposals, most of which involve the attachment of predominantly hydrophobic polymers to surfaces, there is still a lack of covalent bonding to surfaces of antimicrobial agents exhibiting tuneable hydrophilic and fluorophilic chemistries. Satisfied needs

Method used

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  • Antimicrobial substrates and methods of use thereof
  • Antimicrobial substrates and methods of use thereof
  • Antimicrobial substrates and methods of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] This example illustrates a method of making an antimicrobial substrate according to an embodiment of the present disclosure. Allow the 25cm 2 The cotton cloth was stirred for 5 hours. With the resulting acylated cloth at its reflux temperature there is an excess of dehydration-terminated amino-PEG (0.733g, 0.0733mmol, 0.586mmoleq NH 2) ( FIG. 1 ) and 0.8104 g, 10 eq., 5.86 mmol of potassium carbonate in 100 ml of absolute ethanol before stirring overnight, the resulting acylated cloth was washed with chloroform to remove unreacted butyryl chloride. Rinse the resulting cloth with ethanol to remove unreacted reagents before immersing it again in 100 mL of dehydrated ethanol containing 1.216 mL, 10 eq., 5.86 mmol of 1-bromodecane, and 5.86 mmol of potassium carbonate . The mixture was refluxed overnight.

[0104] The next day, rinse the cloth with ethanol again and soak the cloth again in 100 mL of dehydrated ethanol containing 4.159 g, 50 eq., 29.3 mmol of methyl iodi...

Embodiment 2

[0106] In order to test the antimicrobial effect of the antimicrobial substrates of the present disclosure, the following methods can be used.

[0107] Preparation of Escherichia coli (E.Coli) inoculum

[0108] Lysis broth (LB) (1 mL) was added to a 15 ml cell culture tube. Add seven (7) μl of ONE SHOT TM A 40% glycerol stock of TOP10 E. coli cells (Life Technologies, Grand Island, NY) (taken from a -20°C freezer) was inoculated into 1 ml of LB medium. The inoculum was incubated for 3 hours at 37°C with constant shaking (approximately 250 rpm). The inoculum was then centrifuged at 2500 rpm for 4 minutes. The LB medium was separated and the E. coli cells were washed twice with 1 ml of sterile phosphate-buffered saline (PBS) 1X (pH 7.4) each time, and the E. coli cells were resuspended in 1 mL of this buffer. Using densitometry, the final concentration was estimated to be 10 7 -10 8 cells / ml.

[0109] Prepare LB agar medium (1L):

[0110] 800ml of dH 2 O(deionized MILL...

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Abstract

Provided is an antimicrobial substrate comprising a substrate to which is covalently bonded an antimicrobial polymer, in which the antimicrobial polymer comprises an alkylene oxide backbone to which are attached one or more alkyl and / or alkylene oxide primary and / or secondary branches, at least one of the alkyl or alkylene oxide primary and / or secondary branches is functionalized with a quaternary ammonium or a fluorinated group, or at least two of the alkyl and / or alkylene oxide primary and / or secondary branches are functionalized with a quaternary ammonium and a fluorinated group. The antimicrobial substrate can be used, for example, in a method for protecting an object against microbial infection, microbial colonization, or microbial transinfection comprising providing to the object an antimicrobial substrate.

Description

technical field [0001] This disclosure relates in part to antimicrobial substrates and methods of use thereof. Background technique [0002] Pathogenic microorganisms such as bacteria, viruses, fungi, yeasts and algae are known to cause severe illness and death. Microorganisms can be found in many places and objects such as hospitals, gloves, aprons, shields, implants, kitchen stoves, computer keyboards, telephones, factories, animals, food processing equipment, pharmaceuticals, air, water, and agricultural equipment. People, animals, birds and objects that come into contact with these microorganisms are at risk of becoming infected or contaminated by these microorganisms. Therefore, it is necessary to kill or inactivate the above-mentioned microorganisms, or to prevent the above-mentioned surfaces from being attacked by microorganisms. [0003] It has been proposed to modify the surfaces of materials with antimicrobial agents so as to inactivate or kill microorganisms tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01P1/00A01N33/12C08G65/00
CPCA01N33/12A01N25/10A61L2/23C08G65/00C08G65/332C08G65/33303C08G65/337C08G2650/32C08G2650/50C08L2203/02
Inventor 罗杰·A·纳赛尔雪莉·杨马里恩·L·柴尔特罗马克·阿曼
Owner XMICROBIAL
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