Soil conditioner
A technology of soil conditioner and activator, which is applied in the direction of soil conditioning materials, organic fertilizers, fertilizer mixtures, etc. It can solve the problems of long-term action of microbial conditioners, difficult removal of chemical reagents, and high potential risks, and achieve a strong soil base Exchange ability, solve the problem of secondary pollution, good effect
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Embodiment 1
[0050] This embodiment provides a kind of microbial bacterial agent and preparation method thereof, and this method comprises the steps:
[0051] Bacillus subtilis strains were purchased from the Agricultural Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is ACCC11025, the genus name: Bacillus, and the species name epigraph: subtilis.
[0052] 1) Activation of strains
[0053] Inoculate the Bacillus subtilis strains stored at low temperature in LB liquid medium, and cultivate them with shaking at 30°C for 20h-24h, and the obtained fermentation liquid is used as the inoculum;
[0054] 2), the preparation of seed solution
[0055] Add the inoculum solution prepared in step 1) into the LB liquid medium at a volume percentage of 2% to 5%, and shake and culture at 30°C for 12h to 16h to obtain a seed solution.
[0056] 3), fermentation culture
[0057] The seed liquid obtained in step 2) and the fermentation medium a...
Embodiment 2
[0062] This embodiment provides a kind of soil conditioner and preparation method thereof, and this soil conditioner comprises following composition:
[0063] Mineral material, activator A, the microbial inoculum provided in Example 1, activator B and enzyme preparation for providing nutrition for the microbial inoculum;
[0064] The main components of the enzyme preparation are cellulase and urease in an equal mass ratio;
[0065] The mineral material includes 100-200 mesh attapulgite, sepiolite, and zeolite; the mass ratio of the attapulgite, sepiolite, and zeolite is 0.8:0.6:0.8;
[0066] The composition of the activator A is a mixture of citric acid, aminoacetonitrile bisulfate and chelated zinc and boron in a mass ratio of 1:0.01:0.06;
[0067] The mass percentage of the enzyme preparation in the mineral material is 0.05%;
[0068] The activator B includes yeast extract, peptone and amino acid; the mass ratio of the yeast extract, peptone and amino acid is 0.8:1.8:0.8; ...
Embodiment 3
[0074] This embodiment provides a kind of soil conditioner and preparation method thereof, and this soil conditioner comprises following composition:
[0075] Mineral material, activator A, the microbial inoculum provided in Example 1, activator B and enzyme preparation for providing nutrition for the microbial inoculum;
[0076] The main components of the enzyme preparation are cellulase and urease in an equal mass ratio;
[0077] The mineral material includes attapulgite, sepiolite, and zeolite of 100 to 200 meshes; the mass ratio of attapulgite, sepiolite, and zeolite is 1.2:0.4:1.2;
[0078] The composition of the activator A is a mixture of citric acid, aminoacetonitrile bisulfate and chelated zinc and boron with a mass ratio of 1:0.03:0.04;
[0079] The mass percentage of the enzyme preparation in the mineral material is 0.1%;
[0080] The activator B comprises yeast extract, peptone and amino acid; the mass ratio of the yeast extract, peptone and amino acid is 1.2:2.2...
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