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Isolated-shoot rot resistance identification method for fruit trees

A technology of resistance identification and rot disease, which is applied in metal processing and other directions, can solve the problems of low drilling efficiency and uneven hole opening of branches, and achieve high drilling efficiency, uniform hole diameter and wide application Effect

Inactive Publication Date: 2016-09-21
河南省农业科学院园艺研究所
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AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for identification of resistance to fruit tree branch rot disease in vitro, aiming to solve the problems of uneven hole opening and low punching efficiency in the existing method of inoculating fungus with isolated branches

Method used

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  • Isolated-shoot rot resistance identification method for fruit trees

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Embodiment Construction

[0015] In order to further understand the content, features and effects of the present invention, the following examples are given, and detailed descriptions are given below with reference to the accompanying drawings.

[0016] What the invention improves is the punching link of the traditional hole puncher in the identification step of fruit tree branch inoculation disease resistance.

[0017] see figure 1 : The punch includes a fixed pipe and a drill,

[0018] The front end of the fixed pipe is equipped with a drill, and the front end of the drill is provided with a beveled blade.

[0019] The inner diameter of the fixed pipe is 5 mm, and the length of the fixed pipe is 10 mm; the inner diameter of the drill is 4 mm, and the length of the drill is 10 mm.

[0020] The outer wall of the fixed pipe is provided with friction stripes fixed with the electric drill.

[0021] Punching steps:

[0022] 1. Prepare fruit tree branches and electric drills to be drilled;

[0023] 2. ...

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Abstract

The invention discloses an isolated-shoot rot resistance identification method for fruit trees. The punching procedure of a traditional puncher used in the fruit tree shoot vaccination disease resistance evaluation step is mainly improved by a novel puncher which comprises a fixed tube and a borer, the borer is mounted at the front end of the fixed tube, and a beveled edge is formed at the front end of the borer. By application of the isolated-shoot rot resistance identification method, the operation is simple; the diameters of the orifices are uniform; the problem that since orifices formed by a traditional manual borer are inconsistent, experimental errors are caused by sample material vaccination evaluation is solved; the punching efficiency is high; as the edge is driven by an electric drill without manual efforts, the probability of getting fatigue is low, the labor intensity is reduced, and the labor efficiency is improved; the structure is simple; the application is wide; and the isolated-shoot rot resistance identification method for the fruit trees can be widely applied to plant isolated shoot punching vaccination evaluation tests.

Description

technical field [0001] The invention belongs to the field of fruit tree planting, and in particular relates to a method for identifying resistance to fruit tree branch rot disease in vitro. Background technique [0002] Using branches to inoculate fungal (rot disease, ring disease, blight, etc.) mycelium blocks and conidia liquid is a new method for disease identification of horticultural germplasm resources in recent years. A reliable and important method for disease identification. There are two methods of in vivo and in vitro inoculation in this method. According to the literature review, the inoculation test of living branches includes the alcohol lamp burn method and the smash method. These methods are affected by factors such as field conditions, inoculation wound consistency, and time-consuming, so the application is limited. The isolated branch inoculation method is relatively easy to obtain materials, is not limited by field conditions, is relatively easy to carry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B26F1/16B26D7/26
CPCB26F1/16B26D7/2621
Inventor 吴中营王东升郭献平梁慎
Owner 河南省农业科学院园艺研究所
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