A STED super-resolution microscopic system and microscopic method for volume holographic reduction of scanning beam

Abstract

The present invention discloses a STED super-resolution microscopic system and microscopic method for volume holographic reduction of scanning beams. The STED super-resolution microscopic system comprises an excitation light recording device for recording excitation light interference information on a volume holographic material, a consumption light recording device for recording consumption light interference information on the volume holographic material, a scanning light beam reduction device which reduces the light information of the excitation light and the consumption light recorded on the volume holographic material, a polarizing assembly, through which the reduced excitation light and the consumption light become linearly polarized light, a first light beam output assembly for transmitting and reflecting light beams, a two-dimensional scanning galvanometer for swinging the excitation light and the consumption light to realize two-dimensional scanning, a microscope objective lens for imaging a sample, a detection light path, and a display and processing unit for displaying sample images and controlling the two-dimensional scanning galvanometer. The STED super-resolution microscopic system and microscopic method solve the problem that in the existing STED microscopic technology, the coincidence degree of focused light spots between the excitation light and the consumption light is low due to external vibration and temperature difference.

Description

technical field [0001] The invention relates to medical equipment, in particular to a STED super-resolution microscopic system and a microscopic method for volume holographic restoration of scanning light beams. Background technique [0002] Stimulated Emission Depletion (STED) microscopy is based on confocal scanning microscopy, introducing 0 to 2π vortex phase-modulated depletion light to form a hollow spot at the focus and coincide with the excitation spot. Through the principle of stimulated radiation, the fluorescence at the edge of the excitation spot is consumed, and the area of ​​the fluorescence spot is compressed, so that the size of the excitation spot breaks through the diffraction limit and achieves super-resolution. Therefore, STED microscopy, which has the characteristics of far-field super-resolution and tomographic scanning imaging, is widely used in biological sciences and other fields. Since STED microscopy requires that the focused spots of the excitatio...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a STED super-resolution microscopic system and a microscopic method for volume holographic restoration of scanning beams, to solve the following technical problems: 1. The existing STED microscopic technology causes the excitation light Low coincidence with focused spot of light consumption

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