Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antrodia cinnamomea housekeeping gene sequence, qRT-PCR (quantitative real-time polymerase chain reaction) amplification primer, amplification method and screening method

A technology of housekeeping gene and screening method, applied in the field of gene technology, can solve the problem of not finding the housekeeping gene of Antrodia camphorata or internal reference gene, etc., and achieve the effect of reliable results and guaranteed accuracy.

Inactive Publication Date: 2016-08-31
FUJIAN AGRI & FORESTRY UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned patents have not yet found a housekeeping gene or internal reference gene with relatively stable expression of Antrodia Cinnamomea

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antrodia cinnamomea housekeeping gene sequence, qRT-PCR (quantitative real-time polymerase chain reaction) amplification primer, amplification method and screening method
  • Antrodia cinnamomea housekeeping gene sequence, qRT-PCR (quantitative real-time polymerase chain reaction) amplification primer, amplification method and screening method
  • Antrodia cinnamomea housekeeping gene sequence, qRT-PCR (quantitative real-time polymerase chain reaction) amplification primer, amplification method and screening method

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0069] The expression levels of c4163 and c10657 sequences were compared in three different samples. There were three experiments in total, and each group of experiments was repeated three times. The specific reaction conditions of the above three experiments are shown in the following table. Table 3 is the specific reaction condition table of c4163 in the first experiment; Table 4 is the specific reaction condition table of c10657 in the first experiment.

[0070] table 3

[0071]

[0072] Table 4

[0073]

[0074] Table 5 is the specific reaction condition table of c4163 during the second experiment; Table 6 is the specific reaction condition table of c10657 during the second experiment.

[0075] table 5

[0076]

[0077] Table 6

[0078]

[0079] Table 7 is the specific reaction condition table of c4163 in the third experiment; Table 8 is the specific reaction condition table of c10657 in the third experiment.

[0080] Table 7

[0081]

[0082]

[008...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an antrodia cinnamomea housekeeping gene sequence. The housekeeping gene sequence refers to a c4163 or c10657 sequence. The c4163 sequence is shown as SEQ ID NO: 1. The c10657 sequence is shown as SEQ ID NO: 2. The invention further relates to a screening method of the antrodia cinnamomea housekeeping gene sequence, a qRT-PCR (quantitative real-time polymerase chain reaction) amplification primer of the antrodia cinnamomea housekeeping gene sequence and a qRT-PCR amplification method of the antrodia cinnamomea housekeeping gene sequence. The antrodia cinnamomea housekeeping gene sequence can be stably expressed in antrodia cinnamomea samples with different shapes and cultivated in different times, stripe singleness is achieved, and a correction sequence is provided for research on functional gene expression in antrodia cinnamomea.

Description

technical field [0001] The invention relates to gene technology, in particular to antrodia camphorata housekeeping gene sequence, qRT-PCR amplification primers, amplification method and screening method. Background technique [0002] Antrodia cinnamomea is a unique medicinal fungus in Taiwan, my country. It was found to grow on the inner wall of hollow and rotten Cinnamomum kanehirai. Because its host is very small in nature and the growth rate of Antrodia cinnamomea is slow, Antrodia cinnamomea is known as It is known as "the ruby ​​in the forest". As the main active ingredient in the fruiting bodies and mycelia of Antrodia Cinnamomea, triterpenes have many significant effects such as liver protection, detoxification, anti-inflammation, and anti-oxidation. Therefore, the fruiting bodies of Antrodia Cinnamomea are expensive and in short supply. Using the method of molecular biology to increase the content of triterpenoids in the mycelia of Antrodia camphorata becomes mycelia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/31C12N15/11C12N15/10C12Q1/68
CPCC07K14/375C12Q1/6811C12Q2531/113C12Q2527/107C12Q2535/122
Inventor 李晶林雄杰张双双林占熺刘朋虎
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com