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Underground water ecological system health assessment method

An ecosystem and health assessment technology, applied in special data processing applications, instruments, electrical digital data processing, etc., can solve the problems of unsuitable groundwater ecosystem health assessment, low abundance of large organisms, and lack of biological information, etc., to achieve The effect of avoiding errors, strong sensitivity, and broad application prospects

Active Publication Date: 2016-08-17
中科朗劢技术有限公司
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Problems solved by technology

However, due to the low abundance of large organisms and the lack of biological information in the groundwater ecosystem, the biological integrity index method based on the development of producers and consumers is not suitable for the field of health assessment of groundwater ecosystems

Method used

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Embodiment Construction

[0042] In order to describe in detail the technical content, structural features, achieved objectives and effects of the present invention, the following will be described in detail in conjunction with the embodiments.

[0043] Microbial Integrity Index (microbiome index of biotic integrity), hereinafter referred to as M-IBI.

[0044] First, the groundwater around the tailings pond in a city in the Inner Mongolia Autonomous Region was selected, and a total of 12 groundwater samples at different distances from the tailings pond were collected. Select 9 sampling points with obvious human activities, close to the tailings pond, and possible point source pollution as the damaged points (G1, G2, G3, G4, G5, G6, G7, G8, G9), choose far away from the tailings pond, without The remaining groundwater sample points that are polluted by point sources, less affected by human activities, and less polluted are used as reference points (G10, G111, G12). Determination of groundwater pH (pH)...

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Abstract

The invention discloses an underground water ecological system health assessment method. An underground water microorganism integrity index serves as a judgment index. The method includes the following steps that a microbial community richness index is calculated, wherein microbial cells are collected, genome DNA is extracted, PCR amplification is conducted, a target band is cut for purification, and PCR products quantitatively obtained are subjected to paired-end sequencing; candidate biological parameters are determined, wherein a reference point and a damaged point are set, and parameters related to richness in the steps, parameters related to community composition and parameters related to interference resistance are selected as candidate parameters for calculating the biological integrity index; the value of the microbial integrity index is determined, wherein index value distribution range analysis and judgment capacity analysis are conducted, and biological parameters are screened; health detection of an ecological system is conducted by means of 95% quantile optimal value of the microorganism integrity index of the reference point in the steps. The method has high sensitivity and can quickly, sensitively, accurately, comprehensively and objectively reflect health conditions of the underground water ecological system.

Description

technical field [0001] The invention relates to the technical field of environmental pollution monitoring and evaluation, and mainly uses the groundwater microbial integrity index as a judgment index to evaluate the health of the groundwater ecosystem. Background technique [0002] Groundwater is the most abundant and widely distributed freshwater resource on Earth. In recent years, with the rapid development of economy and society, human beings excessively interfere with the groundwater system without protection, resulting in the deterioration of groundwater quality in some areas, the destruction of morphological structure, the change of hydrological conditions, the degradation of habitat and the disappearance of important or sensitive organisms, and even lead to difficult Serious consequences for compensation. Therefore, it is of great significance to carry out research on the health evaluation of groundwater ecosystems, establish effective evaluation indicators and scien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/00
CPCG16Z99/00
Inventor 蔡超安新丽张又弛朱永官
Owner 中科朗劢技术有限公司
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