A fomesafen-degrading bacterium and its application

A technology of fomesafen and degrading bacteria, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of production failure, subsequent crop yield reduction of sensitive crops, pollution of soil environment, etc., and achieve simple cultivation process and low production cost The effect of low cost and broad application prospects

Active Publication Date: 2019-04-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in recent years, the resistance of perennial broad-leaved weeds in soybean fields has increased, and the increase of vicious weeds and the weather have led to a sharp increase in the amount of fomesafen, leaving a large amount of fomesafen in the soil, which not only pollutes the soil At the same time, it has caused critical damage to a variety of sensitive crops that will be stubbled, resulting in reduced or even complete output of sensitive crops that have been stubbled, seriously affecting the adjustment of agricultural planting structures and the safety of agricultural production.

Method used

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  • A fomesafen-degrading bacterium and its application
  • A fomesafen-degrading bacterium and its application
  • A fomesafen-degrading bacterium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Strain isolation and purification

[0033] Soil samples were collected from paddy soil in Jiaxing, Zhejiang Province. Weigh 10.00 g of the tested soil sample into a 100 mL serum bottle, add 1 mL of artificially synthesized Fe(OH) 3 Suspension (15.4 mg / mL Fe content) and 50 mL deionized water were incubated at 30° C. in dark light for 1 week. The cultivated soil suspension was centrifuged at 700 rpm for 10 min, and the supernatant was taken as the microbial inoculum. The inoculum was diluted 100 times, 100 μL was spread on the LB solid medium plate, and the culture dish was sealed with parafilm. Place in a 30°C incubator for 2 days in the dark. Select a plate with uniform colony distribution, pick all single colonies, and culture them in ferric citrate liquid medium. 10mL serum bottles were used as culture bottles, and 5mL ferric citrate liquid culture medium was added to each bottle, flushed with nitrogen gas for 5min, and sealed. If the ferric citrate liquid m...

Embodiment 2

[0037] The effect of pesticide concentration on the degradation of fomesafen:

[0038] In order to study the effect of fomesafen concentration on its own microbial degradation, add 20mL pH 7.0 inorganic salt medium (containing 2.5g / L LB medium and 3.6g / L glucose) to three 25mL sterilized serum bottles respectively , and then each bottle was added fomesafen concentration to 0.5, 1 and 10mg / L, and an appropriate amount of strain FE-1 in the logarithmic growth phase was inoculated in the liquid medium, so that the number of strains reached 10 7 cfu / ml, and then placed in a shaker (30°C, 150rpm) for dark shaking culture, and correspondingly configured 3 blank controls without the bacteria, and the control group was also cultured under the above conditions.

[0039] Samples were taken at regular intervals at 0, 2, 4, 6, 8, 10, 12 and 14 hours of incubation, and the residual amount of fomesafen was detected according to the above method. The experimental group and the control group...

Embodiment 3

[0042] Effect of pH value on microbial degradation of fomesafen:

[0043] In order to study the effect of different pH values ​​on the microbial degradation of fomesafen, 20mL of pH 6.0, 7.0 and 8.0 buffer (containing 2.5g / L LB medium and 3.6g / L Glucose), and then add 10mg / L fomesafen to each bottle. Inoculate an appropriate amount of bacterial strain FE-1 in the logarithmic growth phase in the liquid medium, so that the number of strains reaches 10 7 cfu / mL, and then placed in a shaker (30°C, 150rpm) for dark shaking culture, and correspondingly configured 3 blank controls without the bacteria, and the control group was also cultured under the above conditions.

[0044] Samples were taken at regular intervals at 0, 2, 4, 6, 8, 10, 12 and 14 hours to detect the residual amount of fomesafen. The experimental group and the control group each had three repetitions. The degradation curve of bacterial strain of the present invention to fomesafen under different pH conditions is ...

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Abstract

The invention discloses a Bacillus sp. FE-1 for degrading fomesafen, and its preservation number is CCTCC M 2016044. The fomesafen-degrading bacterium of the present invention has the highest degradation rate of fomesafen under the condition of neutral pH, and the degradation rate gradually increases with the gradual increase of the culture temperature. And the strain has good potential for remediation of contaminated soil. Therefore, the fomesafen-degrading bacteria of the present invention has positive significance for the degradation of fomesafen residues in the polluted environment.

Description

technical field [0001] The invention relates to a bacterium, in particular to a fomesafen-degrading bacterium and application thereof. Background technique [0002] As early as the 1970s, China began to introduce technology to produce and use diphenyl ether herbicides. In the early stage, we mainly used fenfen and glucoquat in rice fields; in the middle and late stages, we mainly started to use Huwei, Kekuol, etc. in soybean fields. With the advancement of domestic herbicide research and development technology and the proficiency in field application technology, in the first few years of this century, due to the further expansion of the planting area of ​​oil crops such as soybeans and peanuts, the weeds and weeds in the field changed. The occurrence of malignant weeds dominated by leaf weeds is becoming more and more serious, resulting in a boom in the production and sales of diphenyl ether herbicides. [0003] Fomesafen [chemical name 5-(2-chloro-4-trifluoromethylphenoxy...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/07A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C12N1/20C12N1/205C12R2001/07
Inventor 虞云龙崔宁
Owner ZHEJIANG UNIV
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