Gold nanoshell-based method for determining capability of antioxidant for clearing H2O2

An antioxidant, H2O2 technology, applied in the direction of chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc., can solve problems such as carcinogenesis, achieve short operation time, wide detection range, preparation handy effect

Inactive Publication Date: 2011-06-22
NANTONG SUZHONG TEXTILE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main drawback of this method is that 9,10-diphenylanthracene used as fluorophore is a carcinogen

Method used

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  • Gold nanoshell-based method for determining capability of antioxidant for clearing H2O2
  • Gold nanoshell-based method for determining capability of antioxidant for clearing H2O2
  • Gold nanoshell-based method for determining capability of antioxidant for clearing H2O2

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Experimental program
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Effect test

Embodiment 1

[0031] A gold nanoshell-based antioxidant for scavenging H 2 o 2 Ability to measure the method, the steps are:

[0032] 1. Incorporation of gold nanoparticles with aminated SiO 2 The colloidal solution is mixed and stirred, and the amount of the added gold nanoparticles should make the gold nanoparticles in the aminated SiO 2 The theoretically calculated value of coverage on the surface of the bead is not less than 30%. For example, 30%, 50%, 70%, and 90% are respectively selected in this embodiment, and the gold nanoparticles are adsorbed on the aminated SiO by electrostatic adsorption. 2 On the surface of colloidal particles, SiO 2 / GNPs composite particle aqueous solution, in the SiO 2 After centrifugation of the aqueous solution of / GNPs composite particles, the unadsorbed gold nanoparticles in the upper layer were removed, and then the SiO 2 / GNPs composite particle aqueous solution was diluted with pure water, and the precipitate was dispersed in pure water to obtai...

Embodiment 2

[0038] 1. Preparation of Gold Nanoparticles (GNPs)

[0039]Gold nanoparticles were prepared by reducing chloroauric acid. Dissolve 1 g of chloroauric acid in 100 mL of pure water to obtain a 1% chloroauric acid aqueous solution, and refrigerate at 4°C. Take 200mL of pre-cooled pure water, add 3mL of 1% chloroauric acid aqueous solution and 1mL of 0.2M potassium carbonate aqueous solution, stir and mix well, then quickly add 9mL of 0.5mg / mL sodium borohydride to it Aqueous solution, the mixed solution changes from dark brown to wine red to obtain gold nanoparticles with a particle size of about 5nm. After stirring for another 10 min, the prepared gold nanoparticle aqueous solution was stored at 4° C. in a refrigerator.

[0040] 2. SiO 2 colloidal amination

[0041] Take 40mL of SiO with a concentration of 0.013g / mL 2 Alcohol sol, add 60μL of γ-aminopropyltriethoxysilane (APTES) to it, stir and react at 70°C for 3h, after the reaction, put the above solution in a centrifuge...

Embodiment 3

[0051] 1. Same as embodiment 2 step 1.

[0052] 2. With embodiment 2 step 2.

[0053] 3. Same as step 3 of embodiment 2.

[0054] 4. Same as step 4 of embodiment 2.

[0055] 5. Tartaric acid scavenge H 2 o 2 Determination of ability

[0056] Select the final concentration of tartaric acid in the reaction system to be 1200 μ M, and all the other experimental operation steps are the same as step 5 of embodiment 2, and the tartaric acid of 1200 μ M is calculated to H 2 o 2 The clearance percentage is 92.5%. Reduce the concentration of tartaric acid so that the final concentrations in the reaction system are 800 μM, 400 μM, 200 μM, and 50 μM, respectively. Repeat the above steps to calculate the 800μM, 400μM, 200μM, 50μM tartaric acid to H 2 o 2 The clearance percentages were 90.1%, 62.9%, 36.7%, 9.1%.

[0057] 6. According to the above experiments, different concentrations of tartaric acid have different effects on H 2 o 2 The clearance rate data, using ORIGIN drawing...

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Abstract

The invention relates to a gold nanoshell-based method for determining the capability of an antioxidant for clearing H2O2, which comprises the following steps: mixing gold nanoparticles (GNPs) with an aminated SiO2 colloidal solution to obtain SiO2 / GNPs composite particles by the action of electrostatic adsorption, wherein the H2O2 can reduce chloroauric acid into gold nanoparticles which depositon the surfaces of the SiO2 / GNPs composite particles to form complete gold nanoshells, and if the antioxidants with different concentrations are firstly mixed with the H2O2 for reaction, after the residual H2O2 reduces the chloroauric acid, only incompletely coated gold nanoshells can be formed; carrying out characterization on the SiO2 / GNPs composite particles and the gold nanoshells with different coating degrees by an ultraviolet / visible light spectrophotometer; comparing the maximum absorption wavelengths of the SiO2 / GNPs composite particles and the gold nanoshells to obtain the clearancerates of the antioxidants with different concentrations on the H2O2; and using the reciprocal of the concentration as the magnitude of the capability for clearing the H2O2. The invention is based on the change of optical properties in the growing process of the SiO2 / GNPs composite particles, and has simple operation and high detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of nano-biological analysis, in particular to a gold nanoshell-based antioxidant for removing H 2 o 2 Ability to measure methods. Background technique [0002] During the metabolic process of the body, reactive oxygen species (ROS) are continuously produced through non-enzymatic and enzymatic reactions. Under normal physiological conditions, the generation and removal of ROS are in a dynamic balance, which can maintain a very low level that is beneficial and harmless. Due to endogenous or exogenous stimuli, the body's metabolism is abnormal and a large amount of ROS is produced, or the body's antioxidant substances are insufficient, which causes the balance between pro-oxidants and antioxidants to be abnormal, which puts the body in a state of oxidative stress, which can oxidize and damage biomolecules. And further cause cell death and tissue damage, which is related to many disease processes. It can be s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 钱卫平马小媛李慧董健
Owner NANTONG SUZHONG TEXTILE
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