Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for extracting β-thymidine from fermentation broth

A fermentation broth, thymidine technology, applied in chemical instruments and methods, sugar derivatives, organic chemistry and other directions, can solve the problems of large safety and environmental protection hidden dangers, complex chemical synthesis processes, and few thymidine extraction processes. High degree of automation, improved extraction yield and guaranteed quality

Inactive Publication Date: 2018-12-04
NANCHANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is the production of thymidine by chemical synthesis. Due to the complexity of the chemical synthesis process, a large amount of organic solvents are needed in the synthesis process, and there are great safety and environmental risks in the production process; the other is the production of thymidine by biological fermentation, but the related thymidine There are still few research reports on the extraction process. Therefore, it is necessary to explore a simple, safe, environmentally friendly and efficient extraction process to provide assistance for the industrial low-cost fermentation of thymidine products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting β-thymidine from fermentation broth
  • A method for extracting β-thymidine from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Heat 100L β-thymidine fermentation broth to 65°C with steam; pass through a microfiltration membrane with a membrane pore size of 1000nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 8 times, add The top wash water volume is 60L. The filtrate passes through the ultrafiltration membrane with a membrane molecular weight cut-off of 3000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the microfiltrate is concentrated by 12 times, 30L of dialysis water is added. The ultrafiltrate passes through electrodialysis equipment at a flow rate of 600L / h to separate anions and cations. When the conductivity of the filtrate is ≤100us / cm, the filtrate is collected for vacuum concentration. Concentrate the filtrate until the content of β-thymidine reaches 30g / L, enter the chromatographic column for separation, collect the chromatographic ...

Embodiment 2

[0025] Heat 100L of β-thymidine fermentation liquid in a fermenter to 65°C by steam; pass through a microfiltration membrane with a membrane pore size of 500nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, the operating temperature is 25°C, and the fermentation liquid is concentrated for 9 After doubling, add 70L of top wash water. The filtrate passes through the ultrafiltration membrane with a membrane molecular weight cut-off of 1000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the ionized liquid is concentrated 15 times, 40L of dialysis water is added. The ultrafiltrate passes through the electrodialysis equipment at a flow rate of 500L / h to separate anions and cations. When the conductivity of the filtrate is ≤100us / cm, the filtrate is collected for vacuum concentration, and the filtrate is concentrated until the content of β-thymidine reaches 35g / L, and then enters the chromatography separation 1. Colle...

Embodiment 3

[0028] Heat 100L of β-thymidine fermentation liquid in a fermenter to 65°C by steam; pass through a microfiltration membrane with a membrane pore size of 200nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C, and the fermentation liquid is concentrated for 6 After doubling, add 60L of top wash water. The filtrate passes through the ultrafiltration membrane with a membrane molecular weight cut-off of 2000, the operating pressure is 1.4 MPa, and the operating temperature is 30-35°C. After the ionized liquid is concentrated by 10 times, 35 L of dialysis water is added. The ultrafiltrate passes through the electrodialysis equipment at a flow rate of 500L / h to separate anions and cations. When the conductivity of the filtrate is less than or equal to 100us / cm, the filtrate is collected for vacuum concentration. The filtrate is concentrated until the content of β-thymidine reaches 40g / L, and then enters the chromatog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting beta-thymidine from fermentation liquid. The method comprises the following steps: (1) preheating the fermentation liquid; (2) performing microfiltration on the fermentation liquid; (3) performing ultrafiltration on the microfiltration liquid; (4) performing electrodialysis on the ultrafiltration liquid; (5) concentrating the electrodialysis liquid; (6) performing chromatography on the concentrated liquid; and (7) concentrating and crystallizing the chromatography liquid. According to the method, a membrane separation technique, an electrodialysis technique and a chromatography separation technique are implemented in the extraction process of the beta-thymidine fermentation liquid, so that a good impurity removal effect can be achieved, and the process is simple, convenient and environment-friendly and high in automation degree. By adopting the extraction process disclosed by the invention, the purity of the liquid phase of a beta-thymidine product is greater than or equal to 98.5%, the external standard content is greater than or equal to 98.5, the total yield is greater than or equal to 70%, industrial application of extracting beta-thymidine from the fermentation liquid is achieved, and good economic benefits and industrial popularization prospects can be achieved.

Description

technical field [0001] The invention relates to a method for extracting β-thymidine from fermentation liquid, belonging to the technical field of biochemical industry. Background technique [0002] β-thymidine, namely 2'-deoxythymidine, is an important precursor for the synthesis of anti-AIDS drugs stavudine, azidothymidine and zidovudine. As the number of AIDS patients in the world increases year by year, the demand for thymidine, the raw material of synthetic anti-AIDS drugs, also increases year by year. [0003] Currently, there are two main methods for producing β-thymidine. One is the production of thymidine by chemical synthesis. Due to the complexity of the chemical synthesis process, a large amount of organic solvents are needed in the synthesis process, and there are great safety and environmental risks in the production process; the other is the production of thymidine by biological fermentation, but the related thymidine There are still few research reports on t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/073C07H1/06
CPCC07H1/06C07H19/073
Inventor 赵大显郑雄敏汪洪袁建赣
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com