A non-invasive online detection device and detection method for the pH of cell culture fluid

Abstract

The invention provides a non-intrusive mode online detection device for a pH value of a cell culture fluid. The non-intrusive mode online detection device comprises an optical path length adjusting unit, a temperature information collecting unit, a sample storage unit and a microprocessor unit, wherein the optical path length adjusting unit is used for adjusting a distance between a light source and a photoelectric sensor; the temperature information collecting unit is used for measuring the cell culture environment temperature; the sample storage unit is provided with at least one to-be-tested sample cabin and an empty cabin; when the to-be-tested sample cabin is located on a measuring position, the light source and the photoelectric sensor are separated by the sample in the to-be-tested sample cabin; when the empty cabin is located on the measuring position, the light source and the photoelectric sensor are communicated with each other; the microprocessor unit is used for acquiring a light intensity signal detected by the photoelectric sensor and an environment temperature collected by the temperature information collecting unit and calculating the pH value of the cell culture fluid. The online detection device and the detection method provided by the invention have the advantages of no damage to samples, zero pollution and economical effect. A visible / near infrared spectroscopic analysis technique is adopted, so that the detection speed is high, the real-time measurement is realized and the precision is high.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, and relates to a non-invasive on-line detection device and method for the pH of a culture solution in a cell culture process. Background technique [0002] Cell culture is a widely used technique in biological and medical research and applications. Using cell culture to carry out in vitro experiments is an important means to explain life phenomena, pathogenesis, screen drugs, prepare vaccines, etc., and is an important part of the medical biotechnology industry. [0003] Cell culture refers to a culture technique in which cells are removed from tissues in the body to simulate the living environment in the body, and grow and reproduce while maintaining structure and function under sterile, appropriate temperature, pH and certain nutritional conditions. [0004] In the process of cell culture, the environment and growth conditions of cell culture (such as medium, buffer environment, g...

AI Technical Summary

Problems solved by technology

[0005] At present, there are three main ways to detect the pH of cell culture fluid: one is to rely on the manual observation of medical technicians, which is intuitive, but the efficiency is low, and it depends on the experience of medical technicians, so it cannot be monitored in real time; the other is Relying on sampling, off-line measurement of pH, etc., the off-line sampling measurement results are relatively accurate, but it often takes time, and due to the limitation of sampling time and times, it cannot guide the control of relevant parameters of cell culture and the optimization of cell culture environment in time; One is to use probes for online detection. This method has a fast measurement speed and can collect data such as cell culture conditions, metabolites, and target product concentrations. However, it is similar to the offline sampling detection method, which is an invasive measurement method that is easy to cause pollution. , will affect the quality of cell culture and increase the cost

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