Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer and method for detecting rectal-cancer-susceptibility-related SNP site

A technology for susceptibility and rectal cancer, applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., to achieve good specificity, good accuracy, and high specificity

Inactive Publication Date: 2016-08-03
CHENGDU ZHONGCHUANG QINGKE MEDICAL LAB CO LTD
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the Chinese patent application number is CN201410452337.6, and the patent name is "Colorectal Cancer Susceptibility Diagnostic Kit and Application of SNP in Its Preparation", which discloses the SNP sites rs12522693 and rs17836917, but the rs3802842 site, rs4939827 site, The rs6983267 locus and rs10795668 locus and the primers for each locus and the method for using these primers to detect SNP loci related to susceptibility to rectal cancer have not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer and method for detecting rectal-cancer-susceptibility-related SNP site
  • Primer and method for detecting rectal-cancer-susceptibility-related SNP site
  • Primer and method for detecting rectal-cancer-susceptibility-related SNP site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Collection of samples and extraction of whole genome DNA

[0035] Use an EDTA anticoagulation tube to collect 3.0 mL of peripheral blood from healthy people (three subjects are randomly selected), and use Tiangen Blood Genome Extraction Kit to extract the subject's whole genome DNA. The operation process is performed according to the kit instructions. The concentration and purity of the whole genomic DNA are detected by an ultra-microprotein nucleic acid analyzer (BioDropμLite), the original data is recorded, and the DNA with the required concentration and purity is stored in the refrigerator at -20℃ for later use.

[0036] 2. PCR amplification, gel recovery, purification and sequencing of the target gene

[0037] PCR amplification of the target gene was performed with the primers at rs3802842 site of C11orf93 gene, the primers at rs4939827 site of SMAD7 gene, the primers at rs6983267 site of POU5F1B gene and the primers at rs10795668 site of RNA5SP229 gene, respectively. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a primer for detecting a rectal-cancer-susceptibility-related SNP site .The primer includes a C11orf93 gene rs3802842 site primer, an SMAD7 gene rs4939827 site primer, a POU5F1B gene rs6983267 site primer and an RNA5SP229 gene rs10795668 site primer .A method for detecting the rectal-cancer-susceptibility-related SNP site with the primer includes the following steps that 1, samples are collected, and DNA is extracted; 2, target genes are subjected to PCR amplification and gel recovery through the four primers respectively; 3, the gel recovery products are subjected to concentration measurement and PCR amplification and purified; 4, the purified products are subjected to sample loading, and the SNP detecting results are analyzed .The primer is good in specificity, high in sensitivity and good in accuracy, the detection method is simple, and the rectal-cancer suffering risk of a detected patient can be forecasted.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a primer and a detection method for detecting SNP sites related to rectal cancer susceptibility. Background technique [0002] Rectal cancer is a common malignant tumor of the digestive tract. Among the tumors of the digestive tract, the incidence of rectal cancer is second only to gastric cancer. The age of onset is mostly between 40 and 60 years old. The average age of onset is 48.3 years. With the development of my country's economy and the improvement of people's living standards, changes in lifestyle and dietary structure, the incidence of rectal cancer is increasing year by year, and the age of onset is gradually getting younger. Therefore, early prediction and prevention and early diagnosis are of great significance to the prevention and treatment of rectal cancer. [0003] The detection of genetic susceptibility genes for diseases is currently a hot spot at home and abro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 陈思翔张蓉刘宇邓银
Owner CHENGDU ZHONGCHUANG QINGKE MEDICAL LAB CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com