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Specific primers and PCR detection method for detecting wheat head blight pathogen fusarium and application of specific primers

A technology for detection of wheat scab and its detection method, which is applied in the field of specific primers for detecting the pathogenic Fusarium of wheat scab, can solve the problems that the species of Fusarium cannot be identified, and achieve specific results, high sensitivity, and operation method and system easy effect

Inactive Publication Date: 2016-08-03
QINGDAO AGRI UNIV
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Problems solved by technology

However, in practical application, the above methods also have certain limitations for the identification of Fusarium species, and Fusarium cannot be identified to species.

Method used

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  • Specific primers and PCR detection method for detecting wheat head blight pathogen fusarium and application of specific primers
  • Specific primers and PCR detection method for detecting wheat head blight pathogen fusarium and application of specific primers
  • Specific primers and PCR detection method for detecting wheat head blight pathogen fusarium and application of specific primers

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Embodiment Construction

[0029] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0030] 1. PCR identification of known strains

[0031] 1. Strain DNA extraction

[0032] Graminea strains, Fusarium oat strains, Fusarium solani strains, Fusarium oxysporum strains and Alternaria strains were used. The above strains were preserved in the laboratory, and commercial strains could also be used.

[0033] 1) Pick the mycelium of the above five strains in PDA medium, culture at 25°C for 4 days, and extract DNA by CTAB method;

[0034] 2) Use a sterilized toothpick to scrape a small amount of mycelium of pathogenic bacteria on the PDA plate, put it in a mortar, grind it with liquid nitrogen to powder, and put it into a 2.0ml centrifuge tube;

[0035] 3) Add 700 μl of 2% CTAB extract, place the centrifuge tube in a water bath at 65°C, shake and mix every 10 minutes, take it out after 60 minutes, a...

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Abstract

The invention provides specific primers and a PCR detection method for detecting wheat head blight pathogen fusarium and application of the specific primers .By carrying out PCR amplification on pathogen DNA with the designed specific primers for wheat head blight pathogen fusarium, a specific fragment with a single strip can be obtained from wheat head blight pathogen fusarium .The detection method is easy to implement, low in time consumption, high in flux and capable of being used for rapidly and accurately detecting wheat head blight pathogen fusarium from wheat ears or seeds suspected to be diseased.

Description

technical field [0001] The invention belongs to the technical field of early warning detection and prevention of crop diseases, relates to a method and application of rapid molecular detection, in particular to a specific primer for detecting wheat scab pathogen Fusarium, a PCR detection method and an application thereof. Background technique [0002] As an important food crop, wheat is widely grown all over the world. In my country, wheat is the second largest grain crop, its planting area and output are second only to rice, and it is also an important commodity grain and a strategic main grain variety in our country. The occurrence of wheat diseases leads to the decline of yield and quality, which brings great difficulties to the production of wheat. Wheat scab (Fusarium headblight, FHB) is one of the three major diseases in wheat production. It has a huge impact on wheat production and is widely distributed. It occurs in wheat producing areas in many countries. It is a w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6895
Inventor 黄金光钱恒伟迟梦宇孙晓梅赵颖
Owner QINGDAO AGRI UNIV
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