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Method for measuring content of glycine betaine in fructus lycii

A determination method and betaine technology, applied in the field of analysis and detection, can solve the problems of weak ultraviolet absorption, cumbersome processing method, low cost of titration method, etc., and achieve the effects of accelerated extraction process, good sample separation, and low test cost.

Inactive Publication Date: 2016-07-27
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each of these methods has its own characteristics. The colorimetric method needs multiple steps such as decolorization of the initial betaine extract and Reye’s salt precipitation method. The processing method is cumbersome and affects the accuracy and repeatability of the measurement results; The interference of the pigment components in the medium makes it difficult to judge the determination end point; the separation effect and method reproducibility in the analysis process of the TLC scanning method are greatly affected by the chromatographic conditions such as developing agent, etc.
The HPLC-DAD detection method is used to determine the content of betaine in Lycium barbarum. Since there is no conjugated system in the structure of betaine and the ultraviolet absorption is weak, it needs to be detected at wavelengths of 192nm and 195nm, and the sensitivity is not ideal.
At present, there is no relatively perfect plan for the research on the detection of betaine content in Lycium barbarum by HPLC-ELSD method

Method used

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  • Method for measuring content of glycine betaine in fructus lycii
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  • Method for measuring content of glycine betaine in fructus lycii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Carry out the determination scheme of the betaine content of Lycium barbarum in the present invention, the Lycium barbarum sample is pulverized with appropriate amount of liquid nitrogen, precision weighing is 1:250 according to mass volume ratio, adds methanol ultrasonic extraction 15min, 0.22 μm microporous membrane filtration, adopts octadecyl Bonded and silica gel packing column, mobile phase is methanol: water (V: V, 20:80), flow rate 1ml / min, drift tube temperature 40°C, spray temperature 40°C, injection 10uL for analysis and determination.

Embodiment 2

[0028] As shown in Example 1, the difference is that the extraction solvent is pure water and methanol-water, and the measurement results find that the peak shape of the chromatographic peak analyzed by the pure water extraction sample is slightly tailed, and the chromatogram obtained by the sample analysis of the methanol-water extraction Peak-to-peak tailing is serious.

[0029] The chromatogram of this embodiment sees figure 1 , figure 2 and image 3 shown, where figure 1 The sample chromatogram is extracted by methanol, and the chromatographic peak shape is good; figure 2 The sample chromatogram is extracted from pure water, and the chromatographic peaks are slightly tailed; image 3 The sample chromatogram is extracted with methanol-water, and the chromatographic peaks are severely tailed.

Embodiment 3

[0031] As shown in Example 1, the difference is that the extraction method adopts reflux extraction, and it was found that ultrasonic extraction is more efficient than reflux extraction. The extraction results obtained by different extraction methods are as follows:

[0032] Table 1 Comparison of extraction effects of different extraction methods

[0033]

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Abstract

The invention discloses a method for measuring the content of glycine betaine in fructus lycii.At present, research on methods for measuring the content of glycine betaine in fructus lycii is increased; however, the methods have defects and disadvantages, the method for measuring the content of glycine betaine in fructus lycii overcomes defects of existing technologies and methods and is complete in extraction, easy and convenient to operate and capable of rapidly and accurately analyzing the content of glycine betaine in fructus lycii.The method mainly includes the steps that 1, a glycine betaine comparison product is extracted, methyl alcohol is added for dissolution, and a comparison product solution is obtained; 2, dried or fresh fructus lycii fruits are taken, a proper amount of liquid nitrogen is added to crush the fructus lycii fruits, methyl alcohol is added for supersonic extraction, and a ample solution is obtained; 3, measuring is conducted according to a high performance liquid chromatography-evaporation light scattering method, an octadecyl key and a silica gel packed column are adopted, the flowing phase is composed of methyl alcohol and water with the volume ratio of methyl alcohol to water being 20: 80, flow velocity is 1 ml / min, the drift tube temperature is 40 DEG C, the atomization temperature is 40 DEG C, the comparison product and the sample solution are taken and fed, and corresponding chromatograph charts are obtained; 4, the chromatograph charts and peak areas corresponding to the comparison product and the sample solution are recorded, the content of the glycine betaine is calculated in a quantitative mode with an external standard method, and the process is completed.

Description

technical field [0001] The invention relates to a method for determining the betaine content of wolfberry, belonging to the technical field of analysis and detection. Background technique [0002] Lycium barbarum (Lycium barbarum L.) is the dry fruit of perennial deciduous shrubs belonging to Solanaceae and Lycium genus. It is a functional and characteristic plant resource with the same source of medicine and food. effect. Lycium barbarum is grown in Ningxia, Xinjiang, Hebei, Inner Mongolia, Qinghai, Gansu and other regions, and the wolfberry produced in Ningxia is the most authentic. There are three main types of active ingredients in wolfberry, and betaine is one of them. Betaine is a kind of quaternary ammonium salt compound, and it is one of the main alkaloids in fruit, leaf and stalk of wolfberry. It has various pharmacological effects such as reducing stress, relieving stress, increasing appetite, and stabilizing vitamins. In addition, because betaine is chemically...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 刘兰英曹有龙韩长锦
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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