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Molecular marker of pear fruit single fruit weight main-effect QTL (quantitative trait loci) site MSF, and application thereof

A molecular marker, single fruit weight technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragment, etc. Effect

Inactive Publication Date: 2016-07-27
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the QTL mapping for pear fruit weight is limited to the parent 'Augusthong', and its practicability is still limited by the F of 'Augusthong' and 'Dangshansuli'. 1 hybrid population

Method used

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  • Molecular marker of pear fruit single fruit weight main-effect QTL (quantitative trait loci) site MSF, and application thereof
  • Molecular marker of pear fruit single fruit weight main-effect QTL (quantitative trait loci) site MSF, and application thereof
  • Molecular marker of pear fruit single fruit weight main-effect QTL (quantitative trait loci) site MSF, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Acquisition of the main QTL site MSF and its molecular marker QS1122-135 for single fruit weight of pear fruit

[0032] (1) 81 F. 1 group;

[0033] (2) Analysis of the two parents and their F 1Population, statistical analysis of genetic types of polymorphic sites between parents in the offspring population, and finally obtained 207 polymorphic marker sites. Then, the linkage analysis of the polymorphic marker loci was carried out using Jionmap4.0 mapping software, and a joint genetic linkage map of 'Hongqili' and 'Wanxiu' was constructed containing 187 SSR markers. Wherein, the experimental procedure of SSR labeling refers to the method of Yomamoto T. et al. (Euphytica, 2002, 124:129-137), and the primers used are synthesized by Jinweizhi Biotechnology Company. SSR markers were detected by 8% polyacrylamide strong base silver staining.

[0034] (3) put F 1 The segregation types of each marker in the population were transformed into lm×ll, nn×np, hk×hk, e...

Embodiment 2

[0042] Example 2: Application test of molecular marker QS1122-135 of the present invention in pear molecular breeding

[0043] (1) Construct F with 'Hongqili' as the female parent and 'Wanxiu Pear' as the male parent 1 generation hybrid population, and obtained 81 strains of F 1 group single plant;

[0044] (2) For the obtained 81 strains of F 1 Single plant carries out QS1122-135 marker detection, and specific method is: use pear genome DNA as template, carry out PCR amplification with the primer pair of molecular marker QS1122-135 as primer, amplified products are separated by 8% polyacrylamide gel electrophoresis ( The result is as figure 2 ); after electrophoresis, determine whether there is a corresponding marker according to the presence or absence of the molecular marker QS1122-135. If there is a marker, it means that the single fruit weight of the single plant is high, and if it does not exist, it means that it is low. The results of molecular marker testing were ...

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Abstract

The invention belongs to the field of pear molecule breeding, and provides a molecular marker of a pear fruit single fruit weight main-effect QTL (quantitative trait loci) site, and application thereof. A starkrimson and Wanxiu pear combined genetic linkage map is built by SSR (simple sequence repeat) marker; the pear fruit single fruit weight phenotype identification is combined; in addition, an interval mapping method is used for QTL analysis; the existence of the main-effect QTL site is detected at the eleventh linkage group of the combined linkage map; the contribution rate of the main-effect QTL site is 51.9 percent. The SSR marker tightly linked with the main-effect QTL site is QS1122-135, and the genetic distance is 4.37cm. The obtained pear fruit single fruit weight main-effect QTL molecular marker can be used for early selection on the basis of fruit characters of single fruit weight; the important theoretical and practice guidance significance is realized for improving the breeding efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding of pears, and in particular relates to a molecular marker of the main effect QTL site MSF of pear fruit single fruit weight, a screening method and application thereof. Background technique [0002] Pear (Pyrus L.) is an important economic crop in my country, and its cultivated area and output both rank first in the world. Pear fruit has high nutritional value, crisp and juicy, sweet and sour taste, and has certain medical value, so it is loved by consumers. Single fruit weight is an important factor for the appearance quality of pear fruit, and the large or small fruit directly determines the yield and economic benefits of pear varieties. Therefore, the single fruit weight of pear fruit is one of the main economic traits of pear varieties. One of the goals of pear breeding is to cultivate a single fruit weight that caters to the needs of different consumers. [0003] Pear is a perenni...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 王龙王磊李秀根薛华柏王苏珂苏艳丽杨健
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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