Alpha-thalassemia gene detecting kit

Abstract

The invention provides a kit capable of detecting four deletion alpha-thalassemia genes (-alpha<3.7>, -alpha<4.2>, --<SEA> and --<THAI>) and three non-deletion alpha-thalassemia genes (alpha<CS>alpha, alpha<QS>alpha and alpha<WS>alpha) at the same time.The kit comprises a DNA chip, a PCR solution I and a PCR solution II.The DNA chip comprises a substrate and probes fixed to the substrate.The kit has the advantages that based on the PCR-reversal point hybridization detecting principle, corresponding amplification primers and probes are designed according to the mutation or deletion loci of genotypes, biotin is used for labeling the primers, amidogen is used for labeling the probes, the DNA chip (nylon membrane) is adopted as a substrate, the probes are fixed to the nylon membrane, and PCR products amplified by the specific primers and the probes fixed to the DNA chip are subjected to hybridization and signal developing box interpretation to diagnose thalassemia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for experimentally diagnosing seven α-thalassemia genes (including 4 deletion-type α-thalassemia genes and 3 non-deletion-type α-thalassemia genes) in clinical samples at one time . Background technique [0002] Thalassemia (referred to as "thalassemia") is a heritable hemolytic blood disease caused by a defect in the globin gene, which reduces or fails the synthesis of globin chains, resulting in an imbalance in the ratio of the globin chains that form hemoglobin. There are two main types: α-thalassemia and β-thalassemia. However, the carrier rate of α-thalassemia is much higher than that of β-thalassemia. [0003] α-thalassemia (including deletion-type α-thalassemia and non-deletion-type α-thalassemia) is one of the most common monogenic genetic diseases in the world. Guangxi, Guangdong, Yunnan, Hainan, Hong Kong and other provinces and cities in southern China and Its surro...

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Problems solved by technology

[0019] The above products and methods all have the risk of missed detection or false detection of non-deletion type α-thalassemia or Thai type α-thalassemia, resulting in the birth of children with moderate to severe thalassemia, which brings a heavy burden to the patient's family

At present, there is no kit that can quickly and easily detect 4 deletions and 3 non-deletions at the same time. It is very inconvenient to use, and it is easy to cause missed detection and cause great harm.

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