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Lung cancer screening kit

A kit and reagent technology, which is applied in the field of lung cancer screening kits and achieves good clinical application prospects.

Active Publication Date: 2016-07-20
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between plasma FABP1 and lung cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Relationship between FABP1 expression level and lung cancer

[0022] The experimental method is as follows:

[0023] 1. Clinical data

[0024] Fifteen patients with early lung cancer and 10 healthy controls were selected. The basic information is as follows:

[0025] Basic Information

lung cancer patients

healthy control

number of people

15

10

age

61.2±12.2

49.5±10.6

male ratio

10(66.7％)

6(60.0％)

[0026] 2. Detection of FABP1 expression level (quantitative detection of FABP1 content)

[0027] Using the AAH-BLG-CUST kit purchased from RayBiotech (Catalog No.: AAH-BLG-CUST), the expression level of FABP1 was detected according to the following method:

[0028] 1. Sample dialysis

[0029] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb t...

Embodiment 2

[0057] Embodiment 2 Composition and method of use of the kit for detecting blood FABP1 expression level according to the present invention

[0058] 1. Composition of FABP1 Detection Kit

[0059] Detection kit (30 persons):

[0060]

[0061] 2. How to use the FABP1 detection kit

[0062] The method of use is as follows:

[0063] 1. Sample dialysis

[0064] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb the supernatant after the second centrifugation, namely plasma, as a test Specimens were stored at -80°C after aliquoting.

[0065] Plasma samples were dialyzed using dialysis tubing prior to biotin labeling. 100 μL of samples were taken respectively and added into dialysis tubes, and then dialyzed in 4000 mL of 1×PBS (pH=8) at 4° C. while stirring. The dialysate was changed every 3 hours, and the samples were collecte...

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Abstract

The invention discloses a lung cancer screening kit, comprising an optional reagent for detecting the expression level of fatty acid binding protein 1 (FABP1). The invention also discloses use of the reagent for detecting the expression level of the FABP1 in preparation of a lung cancer screening reagent. The lung cancer screening kit is capable of being used for judging the risk of lung cancer of people to be detected by detecting the expression level of the FABP1, thus being used for auxiliary diagnosis of clinical lung cancer; the lung cancer screening kit provides an effective basis for taking relevant treatment measures or decisions to a patient; the lung cancer screening kit has good clinical application prospect.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57423G01N33/57484G01N33/68
Inventor 李为民张立刘伦旭刘丹王业唐怀蓉黄燕田攀文朱苡晗谭政堂王彦文
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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