Method for rapidly testing in-vivo absorption of soluble protein of food
A rapid determination and solubility technology, applied in the field of food science and engineering, can solve the problems of less research on protein bioavailability and backward research methods, and achieve the effect of low cost, reliable results and direct process
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Embodiment 1
[0023] Example 1: Determination of protein (polypeptide) running speed in vivo
[0024] The conventional protein is hydrolyzed under the conditions of protease 9600ug, temperature 50 degrees, pH 9.0, and enzymolysis time 60min, and finally the enzymolysis product is obtained. After the product is obtained, it is concentrated to 180ml at about 65°C by using a vacuum evaporator. spare. Take 180-220 grams of rats above the clean level and raise them in a barrier environment as experimental preparation. Three male rats of about 180-220 grams were selected for the experiment, and they were pre-raised for one week before the experiment to allow the animals to adapt to the new environment. The rats were fasted one day before the gastric emptying experiment, and the formal experiment was started the next day. The above-mentioned food soluble protein is quantitatively dissolved in pure water or other solvents, mixed with 0.2ml of colored reagent, and then administered to rats. Afte...
Embodiment 2
[0025] Example 2: In vivo absorption of protein (polypeptide)
[0026] Take rats of 180-220 grams above the clean level, anesthetize, perform abdominal dissection, expose the position of gastric cardia and colon, and perform intubation at both ends. The stomach end cannula was connected to a peristaltic pump, and the protein product produced by enzymatic hydrolysis in Example 1 was pumped in at the flow rate determined in Example 1, and the outflowing liquid was recovered at the end of the colon. According to the difference, the absorption rate of the enzymatic protein was calculated to be 79%.
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