Sinkiang Alaska grayling heritable variation detection method utilizing microsatellite marker Thymalag

A technology of microsatellite marker and genetic variation, which is applied in the field of genetic variation detection of Xinjiang arctic greyfish by using microsatellite marker Thymalag, can solve problems such as restricting the development of molecular genetics, and achieve the effect of simple method.

Active Publication Date: 2016-07-20
广东华轻质量检测服务中心有限公司
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Problems solved by technology

However, due to the lack of microsatellite markers of Xinjiang Arctic grayling, the development of its molecular genetics research is limited, so there is an urgent need to obtain microsatellite markers for the research and application of genetic diversity, individual identification and pedigree authentication of Xinjiang Arctic grayling.

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  • Sinkiang Alaska grayling heritable variation detection method utilizing microsatellite marker Thymalag

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Embodiment Construction

[0023] The following examples describe in detail the present invention's method for molecular genetic markers of Thymalag microsatellite core sequence DNA in arctic grayling in Xinjiang. Firstly, the genomic DNA in the muscle of Xinjiang Arctic grayling was extracted and diluted for later use; then, using the microsatellite core sequence contained in the DNA sequence of Xinjiang Arctic grayling, specific primers were designed at both ends of the sequence; The genomic DNA of individuals in different geographical groups or groups of Xinjiang grayling is amplified by PCR, and the PCR products are detected by denatured polyacrylamide gel electrophoresis to determine the genotype of each individual, that is, to obtain the genetic polymorphism of Xinjiang grayling sex map.

[0024] 1. Extraction of genomic DNA of Xinjiang Arctic grayling: Add 100 μg Xinjiang Arctic grayling muscle into an Eppendorf tube, then add 500 μl of cell lysate and 5 μl of 20 mg / ml proteinase K, shake gently,...

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Abstract

The invention discloses a Sinkiang Alaska grayling heritable variation detection method utilizing a microsatellite marker Thymalag. The method comprises the steps that firstly, a genome DNA in Sinkiang Alaska grayling muscle is extracted and diluted for use; then, a microsatellite core sequence contained in a Sinkiang Alaska grayling DNA sequence is utilized, and specific primers are designed at the two ends of the sequence; then, the primers are used for performing PCR amplification on genome DNA of different geographic stocks or individuals inside Sinkiang Alaska grayling, and a PCR product is detected; strips of the product are analyzed, the gene type of each individual is determined, and a genetic polymorphic map of Sinkiang Alaska grayling is obtained. The method has the advantages that the genetic variation map of the genetic marker Thymalag of Sinkiang Alaska grayling can be obtained rapidly, and the method is simple and convenient to implement.

Description

technical field [0001] The invention belongs to DNA molecular genetic marker technology of Xinjiang arctic grayling (Thymallusarcticusgrubei), and relates to a technical method for detecting Xinjiang arctic grayling microsatellite marker Thymalag, in particular to a genetic variation detection method of Xinjiang arctic grayling microsatellite marker Thymalag. Background technique [0002] Xinjiang arctic grayling (Thymallusarticus grbei) belongs to Salmoniformes, Salmonidae, Thymallinae, Thymallus, commonly known as grass spot, oil spot, also known as stick flower fish, flower fish wings. The body is elongated, flat on the side, the dorsal fin is long and tall, the upper edge is round and convex, and it is in the shape of a flag, with several stripes formed by reddish brown spots, and the body color is bright. Wild resources are mainly distributed in the upper tributaries of the Irtysh River in Fuyun County and Burqin County, Altay City, Xinjiang. Arctic grayling is a typi...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 刘云国
Owner 广东华轻质量检测服务中心有限公司
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