Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of a Molecular Marker in Association Analysis of Milk Yield and Protein Content Traits in Dairy Cows

A technology of protein content and molecular markers, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as not identifying SNPs, achieve shortened generation intervals, low cost, and intuitive pictures Effect

Inactive Publication Date: 2019-11-22
HUAZHONG AGRI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the relationship between this gene and milk production and milk fat percentage, and there are no identified SNPs related to milk production traits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of a Molecular Marker in Association Analysis of Milk Yield and Protein Content Traits in Dairy Cows
  • Application of a Molecular Marker in Association Analysis of Milk Yield and Protein Content Traits in Dairy Cows
  • Application of a Molecular Marker in Association Analysis of Milk Yield and Protein Content Traits in Dairy Cows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Implementation example 1: application example

[0038] An application of a molecular marker MAP4K4 fragment in the correlation analysis of milk yield and protein content traits of dairy cows, the application steps are as follows:

[0039] 1) Primer design:

[0040] According to the partial sequence of the 18th exon of the MAP4K4 gene in the NCBI website (GenBank sequence number AC_000168.1), the applicant designed amplification primers containing the partial sequence, and the primer synthesis was completed by Shanghai Bioengineering Technology Service Co., Ltd. The DNA sequence is as follows:

[0041] P1 forward primer: 5'GGCTCCACTTCACAATACC 3',

[0042] P2 reverse primer: 5'TCTGACTCTGTGCAACCC 3'.

[0043] 2) Purification and sequencing of PCR products:

[0044] PCR amplification reaction system 20ul, including 500ng DNA, 2.0ul 10×PCR buffer, 0.6ul 10mMdNTP, 0.8ul of each primer (10uM) and 1U Ampli Taq DNA polymerase (Shanghai Sangon Bioengineering Technology Servic...

Embodiment 2

[0051] Implementation example 2: association analysis of traits such as dairy cow milk production and protein content related to MAP4K4 gene polymorphism site T156-G156

[0052] After detecting the genotypes of the molecular marker T156-G156 in 413 Holstein dairy cows by PCR-RFLP, statistical methods were used to analyze the correlation between the loci and the milk production and protein content of the cows. Statistical analysis uses R language, using the least multiplication to fit the linear model, the formula is as follows:

[0053] Y ijk =u+F i +P j +SNP k +e ijk

[0054] where Y ijk is the phenotype value, u is the population mean, F i is the site effect results show that P j is a sex effect, the SNP k is the SNP effect, e ijk is a random error.

[0055] Correlation analysis showed that the protein content of individuals with TT genotype was significantly higher (P=0.015) than that of GG genotype, and the milk production of individuals with GG genotype was sig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of preparation of molecular markers of cows, and in particular relates to application of a molecular marker in dairy cow milk yield and protein content trait association analysis. The molecular marker is obtained by cloning from an MAP4K4 gene, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, the molecular marker has a T / G allele mutation at a part of 156bp, and the T / G allele mutation leads to HaeII-RFLP polymorphism. Tests show that the protein content P of an individual having a TT genotype is equal to 0.015, that is to say, the protein content P of the individual having the TT genotype is remarkably higher than that of another individual having a GG genotype; the milk yield P of the individual having the GG genotype is equal to 0.001, that is to say, the milk yield P of the individual having the GG genotype is remarkably higher than those of the individual having the TT genotype and an individual having a TG genotype. The invention also discloses a concrete application method of the molecular marker.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of molecular markers for cattle, and in particular relates to the application of molecular markers in correlation analysis of milk yield and milk protein content traits of dairy cows. The molecular marker is cloned from the mitogen-activated protein kinase kinase kinase 4 (MAP4K4) gene, and is related to milk production and protein content traits of dairy cows. The invention mainly relates to the application of the molecular marker in the correlation analysis of milk yield and milk protein content traits of dairy cows. Background technique [0002] Mitogen-activated protein kinase kinase kinase kinase 4 (mitogen-activated protein kinase kinase kinase kinase 4, MAP4K4) is a member of the serine / threonine kinase subfamily STE20. The gene is mapped to human 2q11.2-q12, and contains a total of 34 exons, and 9 splicing sites are located in the coding region (Becker, et al., 2000). ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张淑君杨利国迪内希任钰为胡林陈星周傲李翔梁爱心
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com