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Aspergillus terreus strain and application thereof in preparing xylnase

A technology of xylanase and Aspergillus terreus, applied in the direction of glycosylase, enzyme, hydrolase, etc., to achieve selective and unique selective effect

Active Publication Date: 2016-07-20
广西多得乐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aspergillus terreus is a commonly used strain for producing lovastatin and phytase in industry, but there are relatively few reports on the production of xylanase by Aspergillus terreus, and xylanase produced by Aspergillus terreus can hydrolyze xylan, and the resulting product Mainly xylobiose has not been reported yet

Method used

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  • Aspergillus terreus strain and application thereof in preparing xylnase
  • Aspergillus terreus strain and application thereof in preparing xylnase
  • Aspergillus terreus strain and application thereof in preparing xylnase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, acquisition and identification of Aspergillus sterreus strain FC7-1

[0033] 1. Obtaining the strain

[0034] (1) Collection of soil samples: The soil in a shallow soil layer of 8-20 cm in a nature reserve in Guangxi, China was collected.

[0035] (2) Isolation and screening of bacterial strains

[0036] 1. prepare screening medium with distilled water; Contain in every liter of screening medium: bagasse xylan 10g, NaNO 3 2g, KH 2 PO 4 1g, FeSO 4 ·7H 2 O0.001g, MgSO 4 ·7H 2 O0.5g, agar 15g; pH7.0; add water to 1L, sterilize with moist heat at 121°C for 20min, mix well, pour plate.

[0037] ②Take 1g of soil sample and put it into a 150mL Erlenmeyer flask, add 19mL of sterile water, stir on a magnetic stirrer for 30min, take the suspension for gradient dilution (10 -1 、10 -2 、10 -3 、10 -4 and 10 -5 ), 100 μL each was spread on a screening medium plate, and cultured at 28°C.

[0038] ③After 5 days, observe the growth of the colonies, sel...

Embodiment 2

[0052] 1. Obtaining xylanase preparation

[0053] (1) Preparation of fermentation medium: the fermentation medium of every L is composed of 5g yeast powder, 5g peptone, 30g corn cob, 0.1gMgSO 4 ·7H 2 O, 0.1g CaCl 2 , 1gKH 2 PO 4 , 1g Tween 80 and water, make up the volume with water.

[0054] (2) 35L fermentation medium was loaded into a 50L fermenter, sterilized at 121°C for 20min, and the Aspergillus terreus (Aspergillusterreus) strain FC7-1 was inoculated into the fermenter at an inoculum size of 1%, fermented at 26°C and 200rpm for 4 days, and fermented During the process, the pH is controlled to be 4.0, and the dissolved oxygen is controlled to be 20%. The xylanase activity of the obtained fermented liquid (ie xylanase preparation) after fermentation is 388U / mL.

[0055] 2. Enzymatic characteristics of xylanase in fermentation broth

[0056] 1. Optimum pH value and optimum temperature of xylanase in fermentation broth

[0057] 1), the most suitable pH value

[00...

Embodiment 3

[0065] Embodiment 3, utilize Aspergillus terreus (Aspergillusterreus) strain FC7-1 to prepare xylanase

[0066] 1. Obtaining xylanase preparation

[0067] (1) Preparation of fermentation medium: the fermentation medium of every L is composed of 10g yeast powder, 10g peptone, 40g corn cob, 0.5gMgSO 4 ·7H 2 O, 0.3gCaCl 2 , 2gKH 2 PO 4 , 2g Tween 80 and water, make up the volume with water.

[0068] (2) 35L fermentation medium is loaded into a 50L fermenter, sterilized at 121°C for 20min, inoculated into the fermenter with an inoculum of 2% of Aspergillus sterreus strain FC7-1, fermented at 28°C and 250rpm for 5 days, and fermented During the process, the pH is controlled to be 5.0, and the dissolved oxygen is controlled to be 30%. The xylanase activity of the obtained fermented liquid (ie xylanase preparation) after fermentation is 263U / mL.

[0069] 2. Enzymatic characteristics of xylanase in fermentation broth

[0070] The optimum action pH value and the optimum action ...

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Abstract

The invention discloses an Aspergillus terreus strain and application thereof in preparing xylnase. The Aspergillus terreus strain FC7-1 is collected under CGMCC No.11657. The invention further provides a method of producing xylnase. The method includes following steps: placing the Aspergillus terreus strain FC7-1 in a fermentation tank for liquid fermentation; collecting a fermentation product, and centrifuging to remove thalli to obtain fermentation supernate which can serve as an xylnase preparation. Optimal pH value of xylnase contained in the xylnase preparation is 5.5, optimal temperature is 55-60 DEG C, and a main product obtained by using the xylnase preparation to hydrolyze bagasse xylan is xylobiose. The Aspergillus terreus strain is of greatly important significance in fully utilizing agricultural waste, increasing comprehensive economic benefit and relieving environmental pressure.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Aspergillus terreus strain and its application in preparing xylanase. Background technique [0002] Xylooligosaccharides are a general term for disaccharides to heptasaccharides connected by xylose units through β-1,4-glycosidic bonds. They are functional oligosaccharides and cannot be digested by the human body. Studies have found that xylooligosaccharides, especially xylobiose in them, can be used as prebiotics to stimulate the growth of probiotics in the human intestinal tract - bifidobacteria, and have a more significant effect than fructooligosaccharides or other oligosaccharides . [0003] At present, the production of xylooligosaccharide mainly includes physical chemical method and biological method. The main process of biological production of xylo-oligosaccharides is to obtain xylan by alkaline extraction, and then use xylanase to degrade xylan to produce xylo-oligosacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/66
CPCC12N9/2482C12Y302/01008C12N1/145C12R2001/66
Inventor 杨齐黄妹平黄斌良吴华德黄燕菲蓝健益
Owner 广西多得乐生物科技有限公司
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