Method for measuring contents of various skin antibacterial agents in wet tissues with ASE-HPLC

Abstract

The invention provides a method for measuring the contents of various skin antibacterial agents in wet tissues with ASE-HPLC to easily, quickly and sensitively detecting various skin antibacterial agents in wet tissues simultaneously.According to the technical scheme, firstly, liquid ingredients in wet tissues are squeezed out, and liquid is collected; secondly, 1 ml of the liquid obtained in the first step is metered and put into a 10 mL extracting pool, static extraction is carried out on the sample with acetone for 3 min at 80 DEG C, extraction is repeated two times, extraction liquid is made to the constant volume of 20 mL with acetone after extraction is finished, 1 mL of liquid is sucked into a centrifugal pipe, swirling is carried out for 2 min, centrifuging is carried out for 3 min at 15000 r / min, and supernate is collected; 3 ul of superanate is taken and injected into a high performance liquid chromatograph for detection, and a chromatogram is measured.The method belongs to the technical field of chemical detection.

Description

Technical field [0001] The invention provides a method for measuring antibacterial agents, specifically, a method for simultaneously detecting the content of multiple skin antibacterial agents in wet wipes; it belongs to the technical field of chemical detection. Background technique [0002] As an emerging sanitary product in recent years, wet tissues have a wide range of uses. They can be used in a variety of personal consumption and commercial occasions, such as civil aviation, homes, hotels, restaurants, and travel. They are gradually being favored by people for their convenience. With the development of people's hygiene habits and the improvement of their quality of life, wet wipes will gradually become a necessity in people's daily life. Wet wipes are made of pure water, spunlace non-woven fabrics and propylene glycol, which are used to wipe face and hands or skin. [0003] The wet wipes on the market can be roughly divided into two categories: One is that it is disinfected ...

AI Technical Summary

Problems solved by technology

[0003] The wet wipes on the market can be roughly divided into two categories: one is to be sterilized itself, but cannot sterilize other items, and contains skin care ingredients, which can only be used for skin moisturizing and maintenance

The other type is disinfectant wipes that are not only disinfected themselves, but can also disinfect other items. They can be used to disinfect or sterilize skin abrasions and scratches. Generally, disinfection or sterilization will be indicated on the packaging ingredients, but the usual antibacterial agents have certain damage to the skin, if used in excess, it will cause serious damage to the skin

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