method for the determination of multiple skin antiseptics in wet wipes by ase‑hplc
A technology of antibacterial agents and wet wipes, applied in the field of chemical detection, can solve problems such as skin damage and inability to sterilize other items, and achieve the effects of quality control, high detection efficiency, and effective analysis
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[0021] A method for ASE-HPLC determination of multiple skin antibacterial agent content in wet paper towels, comprising the steps in turn:
[0022] 1) Squeeze out the liquid components in the wet wipes and collect the liquid;
[0023] 2) Measure 1ml of the liquid obtained in step 1), put it in a 10mL extraction pool, use acetone to extract the sample statically at 80°C for 3 minutes, repeat the extraction twice, and after the extraction is completed, dilute the extract to 20mL with acetone, and draw Put 1mL in a centrifuge tube, vortex for 2min, centrifuge at 15000r / min for 3min, and collect the supernatant;
[0024] 3) Take 3 ul of the supernatant and inject it into a high-performance liquid chromatograph for detection, and measure the chromatogram; the high-performance liquid chromatograph is a Thermo U3000 high-performance liquid chromatograph with a Corona Ultra electrospray detector attached.
[0025] Described chromatographic conditions are as follows:
[0026] The par...
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