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method for the determination of multiple skin antiseptics in wet wipes by ase‑hplc

A technology of antibacterial agents and wet wipes, applied in the field of chemical detection, can solve problems such as skin damage and inability to sterilize other items, and achieve the effects of quality control, high detection efficiency, and effective analysis

Active Publication Date: 2017-06-13
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The wet wipes on the market can be roughly divided into two categories: one is to be sterilized itself, but cannot sterilize other items, and contains skin care ingredients, which can only be used for skin moisturizing and maintenance
The other type is disinfectant wipes that are not only disinfected themselves, but can also disinfect other items. They can be used to disinfect or sterilize skin abrasions and scratches. Generally, disinfection or sterilization will be indicated on the packaging ingredients, but the usual antibacterial agents have certain damage to the skin, if used in excess, it will cause serious damage to the skin

Method used

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  • method for the determination of multiple skin antiseptics in wet wipes by ase‑hplc

Examples

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Embodiment 1

[0021] A method for ASE-HPLC determination of multiple skin antibacterial agent content in wet paper towels, comprising the steps in turn:

[0022] 1) Squeeze out the liquid components in the wet wipes and collect the liquid;

[0023] 2) Measure 1ml of the liquid obtained in step 1), put it in a 10mL extraction pool, use acetone to extract the sample statically at 80°C for 3 minutes, repeat the extraction twice, and after the extraction is completed, dilute the extract to 20mL with acetone, and draw Put 1mL in a centrifuge tube, vortex for 2min, centrifuge at 15000r / min for 3min, and collect the supernatant;

[0024] 3) Take 3 ul of the supernatant and inject it into a high-performance liquid chromatograph for detection, and measure the chromatogram; the high-performance liquid chromatograph is a Thermo U3000 high-performance liquid chromatograph with a Corona Ultra electrospray detector attached.

[0025] Described chromatographic conditions are as follows:

[0026] The par...

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PUM

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Abstract

The invention provides a method for measuring the contents of various skin antibacterial agents in wet tissues with ASE-HPLC to easily, quickly and sensitively detecting various skin antibacterial agents in wet tissues simultaneously.According to the technical scheme, firstly, liquid ingredients in wet tissues are squeezed out, and liquid is collected; secondly, 1 ml of the liquid obtained in the first step is metered and put into a 10 mL extracting pool, static extraction is carried out on the sample with acetone for 3 min at 80 DEG C, extraction is repeated two times, extraction liquid is made to the constant volume of 20 mL with acetone after extraction is finished, 1 mL of liquid is sucked into a centrifugal pipe, swirling is carried out for 2 min, centrifuging is carried out for 3 min at 15000 r / min, and supernate is collected; 3 ul of superanate is taken and injected into a high performance liquid chromatograph for detection, and a chromatogram is measured.The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention provides a method for measuring antibacterial agents, specifically, a method for simultaneously detecting the contents of multiple skin antibacterial agents in wet wipes; it belongs to the technical field of chemical detection. Background technique [0002] As a new sanitary product in recent years, wet wipes have a wide range of uses and can be used in various occasions for personal consumption and commercial use, such as civil aviation, family, hotel, restaurant, travel, etc., and are gradually favored by people due to their convenience. With the development of people's hygiene habits and the improvement of the quality of life, wet wipes will gradually become a necessity in people's daily life. Wet wipes are a kind of paper towels made of pure water, spunlace non-woven fabric and propylene glycol for wiping face, hands, or skin. [0003] Wet wipes on the market can be roughly divided into two categories: one is sterilized itself, but c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/74G01N30/06
CPCG01N30/06G01N30/74
Inventor 陈学松张云平陈启钊赖秀梅
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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