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Relatively enclosed multi-thin layer adherent culture device for production of microalgae

An adherent culture and culture device technology, applied in enzymology/microbiology devices, biochemical cleaning devices, methods of supporting/immobilizing microorganisms, etc. Insufficient and other problems, to achieve the effect of increasing the biomass yield of microalgae, low energy consumption, and improving the efficiency of light energy utilization

Inactive Publication Date: 2016-07-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free CO in liquid 2 The content of the content is very low, resulting in insufficient supply of inorganic carbon, which indirectly affects the carbon sequestration growth of microalgae cells

Method used

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  • Relatively enclosed multi-thin layer adherent culture device for production of microalgae
  • Relatively enclosed multi-thin layer adherent culture device for production of microalgae
  • Relatively enclosed multi-thin layer adherent culture device for production of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The test location is Qingdao, Shandong, and the test time is June 2015. The height of the support is 0.3 meters, and the height of the culture tank is 0.3 meters. Microfiber towels and flocking were chosen as flexible materials to support microalgae on the surface of adherent culture units. A total of 10 adherent culture units were set up in the culture device, including 5 microfiber towels and 5 flocking. Each adherent culture unit is 0.25 meters high and 0.3 meters wide, and the distance between adherent culture units is 0.05 meters. The spirulina is inoculated on the surface of the culture unit of the adherent culture device. The culture fluid in the culture medium storage tank is Zarrouk culture medium (consists of: every 1 liter of culture medium contains 2.5 grams of sodium nitrate, 0.5 grams of dipotassium hydrogen phosphate, 1 gram of sodium chloride, 0.2 grams of magnesium sulfate heptahydrate, sulfuric acid heptahydrate 0.01 g of ferrous iron, 1 g of potass...

Embodiment 2

[0044] The test location is Qingdao, Shandong, and the test time is July 2015. The height of the support is 0.3 meters, and the height of the culture tank is 0.3 meters. Microfiber towels were chosen as the flexible material to support the microalgae on the surface of the adherent culture unit. A total of 12 adherent culture units are set up in the culture device, and each adherent culture unit is 0.25 meters high and 0.3 meters wide, of which 4 adherent culture units have a distance of 0.05 meters (light dilution factor 10×), and the other 4 adherent culture units The spacing is 0.1 m (light dilution factor 5×), and the spacing of the remaining 4 adherent culture units is 0.2 meter (light dilution factor 2.5×). The spirulina is inoculated on the surface of the culture unit of the adherent culture device. The culture fluid in the culture medium storage tank is Zarrouk culture medium (consists of: every 1 liter of culture medium contains 2.5 grams of sodium nitrate, 0.5 grams...

Embodiment 3

[0046] The test location is Qingdao, Shandong, and the test time is July 2015. The height of the support is 0.3 meters, and the height of the culture tank is 0.3 meters. Microfiber towels and flocking were chosen as flexible materials to support microalgae on the surface of adherent culture units. A total of 10 adherent culture units were set up in the culture device, including 5 microfiber towels and 5 flocking. Each adherent culture unit is 0.25 meters high and 0.3 meters wide, and the distance between adherent culture units is 0.05 meters. Inoculate the yellow hair algae (Tribonema sp.) on the surface of the culture unit of the adherent culture device. The culture fluid in the culture medium storage tank is BG11 culture medium (consists of: every 1 liter of culture medium contains 1.5 grams of sodium nitrate, 0.04 grams of dipotassium hydrogen phosphate, 0.0375 grams of magnesium sulfate heptahydrate, 0.036 grams of calcium chloride dihydrate, Citric acid 0.006g, ferric ...

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Abstract

The invention discloses a relatively enclosed multi-thin layer adherent culture device for production of microalgae. The device comprises a bottom bracket, a culture channel, fluid infusion tubes and a culture medium fluid container, wherein the culture channel is fixed above the bottom bracket; the culture channel comprises at least one adherent culture unit, side walls and a bottom plate; each adherent culture unit is generally arranged in the vertical direction; a gap for sunlight penetration is arranged every two adjacent adherent culture units; the side walls, the bottom plate and the culture channel form a structure, only the upper end of which is opened; a position for the input of carbon dioxide is arranged on the culture channel; one fluid infusion tube is arranged above each adherent culture unit; the fluid infusion tubes are communicated with the culture medium fluid container; a through hole for the outflow of culture fluid is formed in the bottom plate of the culture channel; the culture medium fluid container is placed below the through hole so as to collect the culture fluid flowing down from the through hole. According to the device disclosed in the invention, the supply, for algae, of gas-state carbon dioxide is realized, and the production requirement of certain algae which only can utilize free carbon dioxide is satisfied.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a relatively closed multi-layer thin-layer adherent culture device for microalgae production. Background technique [0002] Whether it is the production of high value-added products or energy products, the core of the development of the microalgae industry lies in obtaining biomass on a large scale and at low cost. However, the cultivation efficiency of unicellular microalgae is still far from the requirements of industrialization in large-scale cultivation. [0003] Light energy utilization is the most important factor affecting the growth efficiency of microalgae in large-scale cultivation, and the improvement of light energy utilization efficiency must be solved by optimizing the structure design of the cultivation equipment. The current culture of microalgae, whether it is open pond culture or photoreactor culture, has a common feature of suspension culture in which mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/04
CPCC12M21/02C12M23/34C12M25/06C12M29/06
Inventor 汪辉肖尧刘天中罗国强王俊峰曾娟唐伟伟吴晓娟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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