Immunodetection device and method based on noble electrode steady state electrical state measurement

Abstract

The invention discloses an immunodetection device and method based on noble electrode steady state electrical state measurement.The device comprises a pipeline.The pipeline is divided into a reaction cavity 1, a magnetic separation chamber 2 and an electrical state measurement cavity 3.A magnetic granule reagent bag 4, a sample inlet 5, an enzyme label biological probe reagent bag 6 and a washing liquor bag 7 are arranged on the reaction cavity 1.A substrate reagent bag 8 is arranged on the magnetic separation chamber 2.An electromagnet 9 is arranged on the outer surface of the magnetic separation chamber 2.A non-modified noble electrode 10 composed of a sensitive electrode 19 and a noble layer 20 is embedded on the pipeline wall of the electrical state measuring cavity 3.By adopting the non-modified noble electrode, universality and durability are improved, and the non-modified noble electrode can be used as a fixing assembly of a sensor, so that the purposes of integration / automation of the sensor and low detection cost are achieved; on the basis of reaction solution steady state electrical characteristic measurement of the non-modified noble electrode, solid foundation is laid for detection precision and detection stability.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and relates to an immunodetection device based on the steady-state electrical state measurement of an inert electrode and an immunodetection method based on the device. Background technique [0002] In terms of diagnostics, electroimmunobiosensors have advantages. The core of immune biosensing is to use optical, electrical, magnetic and other physical parameters that can be accurately measured to provide intuitive and quantitative characterization of microscopic immune specific recognition. According to the measurement of physical signals, it can be divided into optical immune biosensors, electrical immune biosensors, magnetic immune biosensors, etc. Among them, compared with optical immune biosensors, electrical immune biosensors can greatly reduce the volume and lay the foundation for portability because there is no restriction on the angle and light path during optical signal excit...

AI Technical Summary

Problems solved by technology

Electrochemical processes cause irreversible changes to the electrode surface and gradually accumulate in the measurement results, affecting the durability of its use

[0006] The above characteristics have caused the following problems in the practical application of electrical immune biosensing: the sensor cannot be integrated / automatically operated, and the electrode, as the core component of the sensor, must be replaced in each detection; the detection cost is increased; each detection Electrodes need to be replaced, and wear and tear will occur during electrode detection. As a result, differences between electrodes and differences in the process of electrode wear greatly reduce the precision and stability of detection.

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