Method for conducting cross breeding through homozygous parent prepared through selfing of bisexual plant and supermale

A technology for hybrid breeding and hermaphrodites, which is applied in the field of asparagus male hermaphrodites self-crossing to prepare homozygous parents and super males for hybrid breeding, which can solve problems such as no public reports and achieve good results and easy operation

Inactive Publication Date: 2016-07-06
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The method provided by the present invention to prepare homozygous parents for hybrid breeding by selfing of hermaphrodite strains, through literature search, there is no public report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Crossbreeding experiments were carried out with the self-bred homozygous offspring of Grande and Diego

[0027] (1) Selection and selfing of hermaphrodites

[0028] Select Grande (Grande) and Diego (Di Aiger) robust, disease-free and harmless male hermaphrodite plants, and select hermaphrodite flowers for selfing and marking according to the selected hermaphrodite plants and their flowering habits. After the selfed flowers bear fruit and mature, the selfed seeds of Grande (Grande) and Diego (Di Aiger) are harvested respectively for preservation.

[0029] (2) Hermaphrodite self-seed sowing and self-purification

[0030] Sow the hermaphrodite seeds obtained by selfing of Grande (Grande) and Diego (Di Aiger), and find the hermaphrodite flowers of the male hermaphrodite plants for selfing when budding or flowering in the adult plant stage, to avoid other flowers. Pollinate and mark well. After the selfed flowers bear fruit and mature, the seeds are harvested, and the ...

Embodiment 2

[0041] 1. Crossbreeding experiments were carried out with the self-bred homozygous offspring of Gulf and Jelim.

[0042] (1) Selection and selfing of hermaphrodites

[0043] Select the healthy, disease-free and harmless male hermaphrodites from Gulf and Jelim. According to the selected hermaphrodites and their flowering habits, select the hermaphrodites for selfing and mark them. After the selfed flowers bear fruit and mature, the selfed seeds of Gelf and Jelim are harvested respectively for preservation.

[0044] (2) Hermaphrodite self-seed sowing and self-purification

[0045] Sow the seeds of the hermaphrodite plants obtained by selfing of Gulf and Jelim, and find the hermaphrodite flowers of the male hermaphrodite plants for selfing when budding or flowering at the adult plant stage, avoid pollination of other flowers, and do a good job mark. After the selfed flowers bear fruit and mature, the seeds are harvested, and the above-mentioned method of sowing and selfing is ...

Embodiment 3

[0056] 1. Crossbreeding experiments were carried out with self-crossed homozygous offspring of Gulf and Diager.

[0057] (1) Selection and selfing of hermaphrodites

[0058] Select the male hermaphrodite plants that are robust, disease-free and harmless from Gulf and Diager, and select hermaphrodite flowers for selfing and marking according to the selected hermaphrodite plants and their flowering habits. After the selfed flowers bear fruit and mature, the selfed seeds of Gulf and Diage are harvested and preserved respectively.

[0059] (2) Hermaphrodite self-seed sowing and self-purification

[0060] Sow the seeds of the hermaphrodite plants obtained by selfing of Gulf and Diager, and find the hermaphrodite flowers of the male hermaphrodite plants for selfing when budding or flowering at the adult stage, avoid pollination by other flowers, and mark them . After the selfed flowers bear fruit and mature, the seeds are harvested, and the above-mentioned method of sowing and se...

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Abstract

The invention relates to a method for conducting cross breeding through a homozygous parent prepared through selfing of a bisexual plant, in particular to a method for conducting cross breeding through the homozygous parent prepared through selfing of an asparagus male bisexual plant and supermale, and belongs to the technical field of cross breeding. The method comprises the implementation steps of selection of the asparagus bisexual plant, flowering observation, and cross breeding, wherein the implementation step of cross breeding comprises the sub-steps of 1, selection and selfing of a bisexual flower; 2, sowing of bisexual flower selfing seeds and selfing purification; 3, hybridization and obtaining of hybridization seeds; 4, management conducted after hybridization; 5, harvesting management. The method has the advantages that the method for conducting cross breeding through the homozygous parent prepared through selfing of the bisexual plant is a new technology used for asparagus cross breeding, and an effective method is provided for utilizing and improving asparagus germplasm resources of China; the method is easy in operation and good in asparagus cross-breeding effect.

Description

Technical field: [0001] The invention relates to a method for cross breeding by selfing hermaphrodites to prepare homozygous parents, in particular to a method for cross breeding by using asparagus male hermaphrodites to prepare homozygous parents and super males, belonging to the technical field of cross breeding. Background technique: [0002] Asparagus officinalis is a monocotyledonous plant with dioecious plants. Generally, female plants of asparagus only have female flowers, and male plants only have male flowers. The stamens in ) degenerated, and the ovary and stigma in the male plant (Mm) stopped growing and differentiating. Therefore, female plants bear fruit and produce seeds, while male plants bear no fruit. In nature, the pistil of some male plants degenerates incompletely and the females recover fertility, which will bear a small amount of fruit and form seeds. Such plants are called male hermaphrodites (genotype is also Mm), commonly known as hermaphrodites. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 刘正杰毛自朝林春张园王乐徐绍忠李和洲
Owner YUNNAN AGRICULTURAL UNIVERSITY
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