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Method for improving imbibition cold damage resistance of plant seeds with N-acylethanolamines

A technology of acylethanolamine and imbibition chilling injury, which is applied to the application of N-acylethanolamine in improving the resistance of seeds to imbibition and chilling injury, and the treatment field of improving the resistance of plant seeds to imbibition and chilling injury, which can solve the problem of low emergence rate and shorten the emergence time. , poor quality of seedlings, etc., to achieve the effect of improving the emergence rate, shortening the emergence time, and improving the ability to resist absorption and cold damage

Inactive Publication Date: 2016-06-29
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The purpose of the present invention is to provide a seed treatment method for improving the resistance of seeds to imbibition and chilling damage, so as to solve the problems of slow emergence, low emergence rate, and poor quality of seedlings caused by seeds encountering imbibition and chilling damage that are ubiquitous in agricultural production. , after exogenous application of N-acyl ethanolamine treatment by the present invention, the ability of seeds to resist imbibition and chilling damage can be improved, the emergence rate can be improved, and the emergence time can be shortened

Method used

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  • Method for improving imbibition cold damage resistance of plant seeds with N-acylethanolamines

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Embodiment 1

[0014] n-butanol treatment system: n-butanol is prepared with deionized water, and the final treatment concentrations are 0.01%, 0.05%, and 0.08%. Control-1, treated directly with deionized water.

[0015] N-acyl ethanolamine (NAE) treatment system: three treatment solutions: (1) Control-1, directly treated with deionized water; (2) Control-2: dimethyl sulfoxide with the same volume as 5 μmol, 10 μmol, and 25 μmol (DMSO), that is, the solubility of DMSO is 0.05%, 0.1%, 0.25%; (3) NAE12:0 is firstly dissolved in DMSO to make a 10 mmol mother solution, and then diluted to the final treatment concentration: 5 μmol, 10 μmol, 25 μmol.

[0016] Seeds sensitive to imbibition and chilling: Cucumber and pea were treated with NAE and n-butanol respectively during imbibition and chilling and germination. Imbibition and chilling were treated at 4°C for 24 hours, then transferred to wet filter paper and placed in a constant temperature light incubator at 25°C. (Day / night photoperiod is 8 / ...

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Abstract

The invention provides a method for improving imbibition cold damage resistance of plant seeds with N-acylethanolamines, belongs to the technical field of crop treatment and particularly relates to a seed treatment method. The method includes the steps that N-acylethanolamines 12:0 is dissolved with dimethyl sulfoxide, the ratio of 12:0 represents the ratio of the carbon number to the double bond number, 10 mmol of mother liquor is prepared, dilution is conducted till the concentration is 5 micromoles to 25 micromoles, and plant imbibition cold damage treatment and exogenous adding treatment after germination are conducted. In the 4 DEG C imbibition cold damage process of the sensitive seeds, N-acyl ethanol is exogenously added, the tolerance of the imbibition cold damage sensitive seeds can be remarkably improved, the crop seeds still have the high germination rate, early seedling development and high seedling development quality when encountering low-temperature attacks, the yield of crops is increased, and good application prospects are achieved.

Description

[0001] This application is a divisional application of the patent application "A Method for Improving Plant Seed Imbibition and Chilling Injury Resistance" (application number: 201510033173.8, filing date: January 29, 2015). technical field [0002] The invention belongs to the technical field of crop treatment, and specifically relates to a treatment method for improving the resistance of plant seeds to imbibition and chilling damage, and more specifically relates to the application of N-acylethanolamine in improving the resistance of seeds to imbibition and chilling damage. Background technique [0003] Seeds occupy a special and important position in agricultural production. To turn the potential of seeds with high-yield and high-quality genetic characteristics into real productivity, the most important link in cultivation is to maintain high seed vigor and obtain full and strong seedlings. However, many cultivated crops suffer imbibition and chilling damage during the ini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01N37/20A01P21/00
CPCA01C1/00A01N37/20
Inventor 李唯奇禹晓梅
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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