A method for improving the resistance of plant seeds to imbibition and chilling damage
A technology for imbibition and chilling damage and plant seeds is applied in the treatment of improving the resistance of plant seeds to imbibition and chilling damage. The application field of n-butanol and N-acylethanolamine in improving the resistance of seeds to imbibition and chilling damage can solve the problem of shortening the emergence time, Problems such as poor quality of seedlings and low emergence rate can shorten the emergence time, improve the ability to resist imbibition and cold damage, and improve the emergence rate.
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[0015] n-butanol treatment system: n-butanol is prepared with deionized water, and the final treatment concentrations are 0.01%, 0.05%, and 0.08%. Control-1, treated directly with deionized water.
[0016] N-acyl ethanolamine (NAE) treatment system: three treatment solutions: (1) Control-1, directly treated with deionized water; (2) Control-2: dimethyl sulfoxide with the same volume as 5 μmol, 10 μmol, and 25 μmol (DMSO), that is, the solubility of DMSO is 0.05%, 0.1%, 0.25%; (3) NAE12:0 is firstly dissolved in DMSO to make a 10 mmol mother solution, and then diluted to the final treatment concentration: 5 μmol, 10 μmol, 25 μmol.
[0017] Seeds sensitive to imbibition and chilling: Cucumber and pea were treated with NAE and n-butanol respectively during imbibition and chilling and germination, treated with imbibition and chilling at 4°C for 24 hours, then transferred to wet filter paper, and placed in a constant temperature light incubator at 25°C (Day / night photoperiod is 8 / ...
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