SNP detecting kit for methionine-folic acid metabolism key enzymes MTHFR and use method thereof
A detection kit and folic acid metabolism technology, applied in the field of medical detection, can solve the problems of unsuitability for rapid clinical detection, difficult clinical promotion, and long operation time, and achieve the effects of shortening detection time, easy judgment, and intuitive judgment.
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[0069] As an improved specific embodiment, the volume ratio of the primer and the probe is 1:1.
[0070] As an improved specific embodiment, the amounts of the forward primer and the reverse primer are the same.
[0071] As an improved specific embodiment, the MTHFRC677T wild-type probe and the MTHFRC677T mutant probe are used in the same amount.
[0072] As an improved specific embodiment, the MTHFRA1298C wild-type probe and the MTHFRA1298C mutant probe are used in the same amount.
[0073] The method for using the kit comprises the following steps:
[0074] 1) Extract the genomic DNA of the person to be tested;
[0075] 2) Each PCR system includes volume parts: 10-25 parts of master mix, 1 part of DNA template, 1-2 parts of primers, and 1-2 parts of probe;
[0076] 3) Put the configured PCR system into a fluorescent quantitative PCR instrument for Real-time PCR detection; the reaction conditions are:
[0077] Pre-denaturation at 95°C for 10 minutes; cycle stage, 95°C for...
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