Kit for detecting human ABCC4 gene polymorphism and application thereof

Abstract

The invention relates to a kit for detecting polymorphism of a human ABCC4 gene and an application thereof. Substances in the kit comprise a specific primer and a specific fluorescent probe for detecting an rs868853 SNP locus on the ABCC4 gene, Taq DNA polymerase, a dNTP mixed liquid, an MgCl2 solution, a fluorescence quantitative PCR reaction buffer solution and deionized water. The defects of various conventional genetic mutation detection methods are overcome. The kit has the advantages of high detection accuracy, simple and convenient detection, short detection time, and simple result analysis, is suitable for use in clinical laboratories, can perform qualitative detection on a CYP2C19 gene polymorphism locus, performs PCR fluorescent amplification reaction according to the SNP locus, can judge a result just according to a condition whether two different fluorescence curves are started or no, cannot produce artificial errors, has low false positive and false negative rates, improves the detection accuracy, and reduces detection costs.

Description

technical field [0001] The invention relates to the gene detection technology in the clinical detection technology in the field of biomedicine, in particular to a kit for detecting human ABCC4 gene polymorphism and its application. Background technique [0002] ABCC4 (ATP-bindingcassettetransporterfamilyclassC4, ABCC4) is a member of the ABC protein family, located on chromosome 13q32, 312kb long, contains 31 exons, and encodes 1325 amino acids [6]. The structure of ABCC4 has a certain specificity in the ABCC family. It lacks a transmembrane domain at the N-terminus and has only two transmembrane domains, forming the structure of MSD1-NBD1-MSD2-NBD2, which is the simplest structure in the ABC family. protein. ABCC4 is mainly involved in biological functions such as transporting organic anions produced in the body's substance metabolism and some heterogeneous biomass. The SNP polymorphism of rs868853 on the ABCC4 gene mainly involves antiretroviral drugs, antibiotic drugs, ...

AI Technical Summary

Problems solved by technology

Gene sequencing is currently the most widely used and more accurate method, but this method requires expensive equipment and professional personnel to operate, time-consuming and laborious, and it is difficult to promote in clinical practice

The gene chip method has the advantages of fast and high throughput, but the operation is complicated, there are many steps, the cost is high and it is not easy to popularize

The method of detecting gene mutation with restriction fragment length polymorphism (RFLP) has the advantage of low cost, but the process is cumbersome and takes a long time and is not suitable for popularization

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