Extraction method of deoxydihydroxanthoangelol H chalcone suitable to serve as liver cancer targeted drug
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A technology for extracting deoxygenated dihydroyellow and its extraction method is applied in the field of extracting deoxygenated dihydrogenated yellow angelica alcohol H chalcone, which can solve the problems of single chalcone and the application of the preparation method that have not been reported, and achieve high product purity, The effect of simple process
Inactive Publication Date: 2016-06-15
QINGDAO UNIV
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In the existing technology, the obtained chalcone product is the whole ketone product of Ashitaba, but there are known 8 kinds of chalcone derivatives in Ashitaba, a single chalcone, especially deoxydihydrogen yellow The preparation method of angelica alcohol H chalcone and its application in the treatment of liver cancer have not been reported at present
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Embodiment 1
[0045] The extraction method, the steps are as follows:
[0046] The first step is to beat 1Kg of fresh Japanese leaves into a slurry, mix the slurry with the alcohol-water system of 5:1 (V / V) in the ratio of 1:6, heat up to 70°C, stir and leach for 24 hours, and then filter , to obtain filtrate A6.5L.
[0047] In the second step, mix A and petroleum ether at a volume ratio of 1:1, stir at room temperature for 10 h, let stand to separate layers, remove chlorophyll impurities, and take the hydroalcoholic phase B6L.
[0048] In the third step, replace the silica gel column on B for 3 hours, wash with water to remove water-soluble small molecules such as sugars, and finally elute with a mixed solvent of n-hexane: ethyl acetate = 1:1 to obtain 3.6 L of filtrate, evaporate and dry. 18.3 g of deoxygenated dihydroangelinol H chalcone crude product was obtained.
[0049] In the fourth step, C was dissolved in methanol and recrystallized twice to obtain 10.2 g of refined deoxydihydro...
Embodiment 2
[0051] Except that n-hexane:ethyl acetate=1:3, and the number of recrystallization times is 3 times, the rest are the same as in Example 1.
[0052] Explanation: 9.3 g of deoxydihydroflavored angelica alcohol H chalcone refined product was obtained, and the extraction rate was 0.93%.
Embodiment 3
[0054] Except n-hexane:ethyl acetate=5:1, the others are the same as Example 1.
[0055] Explanation: 11.5 g of the obtained deoxydihydroflavored angelica alcohol H chalcone refined product, the extraction rate is 11.5%.
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Abstract
The invention discloses an extraction method of deoxydihydroxanthoangelol H chalcone suitable to serve as a liver cancer targeted drug. The preparation process consists of: alcohol-water extraction of Angelica keiskei, removal of chlorophyll with petroleum ether, silicagel column adsorption of deoxydihydroxanthoangelol H chalcone, water elution of saccharides and other water-soluble impurities, elution of deoxydihydroxanthoangelol H chalcone with a mixed solvent of n-hexane and ethyl acetate, and secondary recrystallization with methanol, thus obtaining a deoxydihydroxanthoangelol H chalcone refined product. The method provided by the invention has a simple and easily controllable process, and the obtained deoxydihydroxanthoangelol H chalcone product has high purity. Living animal contrast experiments show that: the Angelica keiskei deoxydihydroxanthoangelol H chalcone prepared by the method provided by the invention has a significant inhibiting effect on liver cancer intercellular space blood vessel growth, promotes cancer cell necrosis, is accord with the targeted drug characteristics, can be used as a liver cancer tumor angiogenesis inhibitor, and is suitable to serve as a liver cancer targeted drug.
Description
technical field [0001] The invention relates to a method for extracting chalcone from Ashitaba, in particular to a method for extracting deoxydihydroflavored angelica alcohol H chalcone suitable for use as a liver cancer targeting drug. Background technique [0002] Cancer has become the health killer of all human beings in the 21st century, and it is also one of the major scientific and technological problems that governments and scientists have invested a lot of money and manpower to solve. [0003] According to the 2012 data released by the National Cancer Registry Center, there are about 3.5 million new cancer cases in China every year, and about 2.5 million people die from it. The probability of suffering from malignant tumors in a person's life is 22%. In addition to living habits and environment, diagnosis and treatment are very important. [0004] Among the four types of malignant tumors including liver, esophagus, stomach and lung, China ranks first in the world in...
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