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Human adipose derived mesenchymal progenitor cells for treating hepatitis B

A progenitor cell and adipose technology, applied in the field of adipose stem cells for the treatment of hepatitis B

Inactive Publication Date: 2016-06-15
CELLULAR BIOMEDICINE GRP SHANGHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, there is still a lack of a method in this field that can effectively treat hepatitis B, especially the method that can completely remove hepatitis B virus and repair liver cell necrosis caused by hepatitis.

Method used

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  • Human adipose derived mesenchymal progenitor cells for treating hepatitis B
  • Human adipose derived mesenchymal progenitor cells for treating hepatitis B
  • Human adipose derived mesenchymal progenitor cells for treating hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060] In the present invention, the preparation method of adipose mesenchymal progenitor cells may include the steps of: washing adipose tissue, then digesting it with collagenase, centrifuging to separate stromal vascular components, removing oil and collagenase, culturing primary cells, and obtaining passaged adipose tissue mesenchymal progenitor cells.

[0061] Antigen detection of adipose-derived mesenchymal progenitor cells

[0062] The adipose mesenchymal progenitor cells used with the present invention are of high purity and are substantially free of other types of cells or stem cells. This can be verified by detection of cell surface antigens.

[0063] Adipose mesenchymal progenitor cells have a variety of specific antigens and receptors, mainly CD3, CD13, D29, CD34, CD45, CD49e, CD59, CD73, CD90, CD105, HLA-ABC, etc.

[0064] CD34 antigen is a highly glycosylated type I transmembrane protein, which is selectively expressed on the surface of human hematopoietic stem...

Embodiment 1

[0085] Example 1 Culture of adipose mesenchymal progenitor cells

[0086] 1. Sterile surgical instruments and consumables

[0087] (1) 5 sterile long-handled surgical forceps

[0088] (2) Sterile 100 mesh filter

[0089] (3) Sterilized 40 mesh filter

[0090] (4) 50ml centrifuge tube

[0091] (5)T175, T75 culture flask

[0092] (6) T10ml, T25ml pipette

[0093] (7) Wide mouth pipette

[0094] 2. Sterile reagents:

[0095] (1) MSCSFM medium (life)

[0096] (2) Type I collagenase (prepared and used now): 0.1% collagenase I preparation method: Weigh 0.1 g of collagenase I powder and dissolve it in 100 ml of medium without any factor, and preheat at 37°C before use.

[0097] (3) Sodium chloride injection

[0098] (4) 0.125% Trypsin-0.01% EDTA solution

Embodiment approach

[0100] 1. To receive the adipose tissue, wipe the outer wall of the container containing the adipose tissue with 75% alcohol;

[0101] 2. Aliquot adipose tissue, each T175 culture flask is divided into 50ml of adipose tissue. 10ml pipette, remove the pipette tip, first suck up the lower layer of red liquid in the fat collection bottle and discard it, and then mix the remaining upper layer of fat before packing.

[0102] 3. Wash the adipose tissue to remove blood cells. Add 100ml of sodium chloride injection to the T175 culture flask, tighten the lid, shake vigorously for 3 minutes to fully wash the adipose tissue, then stand for 3-5 minutes to separate the different phases, and suck off the lower aqueous phase; repeat the above operation three times until The lower liquid became clear.

[0103] 4. Collagenase I digestion: add an equal amount of newly prepared collagenase I solution (preheated on an air-bath shaker at 37°C for half an hour in advance), seal with parafilm, shake...

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Abstract

The invention relates to application of human adipose derived mesenchymal progenitor cells in preventing or treating hepatitis B. Specifically, the human adipose derived mesenchymal progenitor cells can be used for preparing a pharmaceutical composition for treating the hepatitis B. The pharmaceutical composition, when applied to an object in need, can improve such indexes as hepatitis B surface antigen, hepatitis B surface antibody, hepatitis B virus e antigen, hepatitis B virus e antibody, hepatitis B core antibody and the like, and can significantly reduce hepatitis virus DNA and lower ALT, so that the repair of hepatic function is promoted.

Description

technical field [0001] The present invention relates to the application field of adipose stem cells, in particular, the present invention provides the use of an adipose stem cell for treating hepatitis B. Background technique [0002] Liver disease is divided into viral liver disease and non-viral hepatitis. Viral hepatitis mainly includes A, B, C, D, and E viral hepatitis, and non-viral liver disease mainly includes alcoholic liver disease, drug or poison-induced liver disease, abnormal metabolism liver disease, and fatty liver disease. Chronic hepatitis B has a global distribution and can lead to diseases including liver decompensation, cirrhosis, and hepatocellular carcinoma. Active hepatitis B virus (HBV) replication is a major driver of liver injury and disease progression. [0003] Severe hepatitis B is a severe hepatitis caused by infection with HBV. Its onset is dangerous and progresses rapidly. Most patients have a poor prognosis. Large areas of hepatocyte necrosi...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P1/16A61P31/20
Inventor 曹卫张丽戴成祥刘佳蔡松柏郑成小
Owner CELLULAR BIOMEDICINE GRP SHANGHAI
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