Method for detecting iodide ions by unmarked fluorescent probe based on thioflavin T dye design

Abstract

The invention provides a method for detecting iodide ions by an unmarked fluorescent probe based on thioflavin T dye design. The method comprises the steps of adding a buffer solution into the prepared thioflavin T / deoxyribonucleic acid (DNA) / Hg<2+> unmarked fluorescent probe, and adding iodine ion solutions with different concentrations into reaction liquid for carrying out a reaction; measuring a fluorescence value of the mixed liquid, and establishing an equation for detecting the solubility of the iodide ions in unknown liquid under test. After the method is used, the currently common detection mode for detecting the iodide ions is changed, and the iodide ions are detected by an unmarked fluorescent probe technology for the first time, so that the defects that the existing detection method is low in sensitivity and complicated in operating steps, needs expensive large-scale instruments, is difficult in realization of rapid on-line detection, and the like can be overcome; the method has the advantages of being rapid in detection response, high in sensitivity, good in selectivity and the like, is a proper, effective and practical detection method, and can be applied to determination of the iodide ions in practical samples.

Description

technical field [0001] The invention belongs to the technical field of anion detection, and in particular relates to a method and application of an unlabeled fluorescent probe designed based on thioflavin T dye to detect iodide ions. Background technique [0002] The identification and detection of anions plays an important role in the fields of biosensing, chemical analysis, and environmental detection. Among many anions, iodide ion has received extensive attention because of its important biomedical significance. Iodine is one of the essential trace elements for the human body, which plays a vital role in normal human growth, neurodevelopment and thyroid function. However, abnormal iodine ion concentration levels are usually associated with diseases, and iodine deficiency or iodine excess can lead to diseases such as goiter, hypothyroidism, and hyperthyroidism, which affect human health [Li, Y.J., Tseng, Y.T. , Unnikrishnan, B., Huang, C.C., 2013. ACS Applied Materials I...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for detecting iodide ions based on an unlabeled fluorescent probe designed based on thioflavin T dye, to overcome the high cost, complicated operation and need for expensive large-scale instruments in the existing methods for detecting iodide ions, which are difficult to realize Quick online detection, not conducive to large-scale promotion of defects

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